TFome Information for pUT5553

TFome Name:pUT5553
Genbank Insert ID:KJ727627
Protein Name:ZmbZIP45
Species: Maize
TF Family: bZIP
Template: BT060756
Corresponding TF Gene: GRMZM2G093020
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B809_F 3' Primer Name: B809_R
5' Primer Sequence: caccATGGTAGCACTGAACATGACCAG 3' Primer Sequence: GAAGCACTGGTGCAACAAGTGGT
5' Primer Tm (º C): 61.1 3' Primer Tm (º C): 60.4
PCR Condition: Volumes per reaction (25l): 5 l GC buffer (Thermo Fisher) 1 l primer forward 20M 1 l primer reverse 20M 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (Thermo Fisher) 9.75 l ultrapure water Initial PCR program: 40 sec 98°C 33x : 15 sec 98°C 30 sec 60°C 30 sec 72°C 7 min 72°C leave at 10°C
Nucleotide Sequence: ATGGTAGCACTGAACATGACCAGCCAGAACTACGCGGCAGCAGCAGCTCAGAACTCGGTGCTGCGCACCCAGATGATGGA
GCTGCAGAGCAGGCTCTGCGCACTGCGTGAGATCATATGCCATATGTACGCAACAACAACGAATGTCCATCCAGCAACTG
TCATGGGCGGTGCTGCTACCTACCATACTTTTGGCGAAACTGCGTGGAACTCTGGCAGCTGCAGCATGCAAATGGTCGAC
CACTTGTTGCACCAGTGCTTC
TFome clones do not contain stop codon at 3'end.
Translation: MVALNMTSQNYAAAAAQNSVLRTQMMELQSRLCALREIICHMYATTTNVHPATVMGGAATYHTFGETAWNSGSCSMQMVD
HLLHQCF
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map