TFome Information for pUT5680

TFome Name:pUT5680
Genbank Insert ID:KJ727739
Protein Name:ZmbZIP43
Species: Maize
TF Family: bZIP
Template: BT069623.1
Corresponding TF Gene: GRMZM2G149150
Transcript: T01
Vector: pENTR/SD/D-TOPO
Note: c.G109A p.A37T Present in template FlcDNA.

5' Primer Name: B514_F 3' Primer Name: B514_R
5' Primer Sequence: caccATGGGTGACCCGAGCGC 3' Primer Sequence: CGCCGACTTCTGCGGCTGTT
5' Primer Tm (º C): 70.5 3' Primer Tm (º C): 68.6
PCR Condition: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGGTGACCCGAGCGCCTCCGCCGACCCCGCCGCCGCGCGCCACCACCTCTCGCCACAGCTCGCGGGGCAGCCGCCGGT
GCCGCGCTCGCCGGCCCCGCTCGACCTGACCTCCGCCGCCGCGGCGTCCGGGCACCGCCGCCTCTCGCCGTCGCTCCGGC
CGCCGGCGCACCCGCAGGCGCGCCTCCCGTCGCCCTACGGCCAGATCTCTTCGCCAGGCGCCGCCGCGCACCACGCGCTC
TCGCTCTCCCAGCCGCTCTTCTTCTCGCTCGACTCGCTCCCGCCGCCGCCGCCGCCGTACGCTGATCTGGGCGCCGCGCC
CGCCGTGCCGGCGCCCTCCCCGCCGCCGTCGACCTCGGACCCGCCGCCGCCGCTCGGCCTGCCGCCCAGGAGGTCCGGCC
ACCGCCGCTCGCAGAGCGACATCCCCTTCGGCCTCGCGCAGCTCAGCCCGCCGCTGCCCCCGCCGGCACCGGTCAAGCGC
GAGGTCGCCACTGCGGCCGAGGGCGGCGACGGCGATGACGCCGCCTTCTTCGACCTCGTCAACGCCTACATGGACCTGGA
CGGTCTGGACCCGCTCAACTCCTCTGAGGACCGCCACGACGACCGCGACAGCCGCGCCAGCGGCACCACGCGCGCCGGGA
GCGCGGCGGAGAGCAGCGAGAACGAGGCGGAGAGCCAGTCCACGTCGGCGGACAGGAAGGACGGGGGCAAGTCCCGCCAT
TGTCGCAGCCTGTCCATGGACAGCTTCATGGGCAAGCTCAACTTCGCCGCAGGGGATGAGTCTCCAAAGCTGCCGCTGCC
GTCTCCTCGTGCCAGTGGCAGCCTCACTAGGAGCGGGAGCGGCTCACTGGAAGGTGGTGCTGTCGCGCTGTTTGACATGG
AGTTCTCCAATGGAGAGTTTACTGAGTCCGAGAAGAAGAAAATCATGGCGAATGAGCGGCTTGCTGAGATAGCTTTGACG
GATCCTAAGAGGGTCAAGAGGATTCTGGCAAATCGGCAGTCAGCAGCAAGGTCAAAGGAGCGCAAGATGAGGTACATTCA
GGAGCTTGAGCATAAGGTGCAAGTGCTGCAGACAGAGGCTACGACACTCTCAGCACAGTTGACAATGCTGCAGAGGGACT
CAGCTGGACTGGCTACTCAGAACAACGAGTTGAAAATTAGGCTGCAAGCCATGGAGCAACAAGCACAGCTGAGAGATGCC
CTGAATGAAGCGTTAACGGGCGAGGTCCAGCGTCTGAAACTCGCAACTGGGGAGATGACCGATGGGCCTATGCCGAAGGG
CCTGCAGCAGCAGATGAACTCCCAGATGCTGCAGCTCCAGCAGCTGCAGATCCAGCAGCAGGCGCCCCCACAACAGACGC
AGCAGCAAGGCCAGCGACAACAGCCGCAGAAGTCGGCG
TFome clones do not contain stop codon at 3'end.
Translation: MGDPSASADPAAARHHLSPQLAGQPPVPRSPAPLDLTSAAAASGHRRLSPSLRPPAHPQARLPSPYGQISSPGAAAHHAL
SLSQPLFFSLDSLPPPPPPYADLGAAPAVPAPSPPPSTSDPPPPLGLPPRRSGHRRSQSDIPFGLAQLSPPLPPPAPVKR
EVATAAEGGDGDDAAFFDLVNAYMDLDGLDPLNSSEDRHDDRDSRASGTTRAGSAAESSENEAESQSTSADRKDGGKSRH
CRSLSMDSFMGKLNFAAGDESPKLPLPSPRASGSLTRSGSGSLEGGAVALFDMEFSNGEFTESEKKKIMANERLAEIALT
DPKRVKRILANRQSAARSKERKMRYIQELEHKVQVLQTEATTLSAQLTMLQRDSAGLATQNNELKIRLQAMEQQAQLRDA
LNEALTGEVQRLKLATGEMTDGPMPKGLQQQMNSQMLQLQQLQIQQQAPPQQTQQQGQRQQPQKSA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map