TFome Information for pUT5685

TFome Name:pUT5685
Genbank Insert ID:KJ727744
Protein Name:ZmARID5
Species: Maize
TF Family: ARID
Template: RT-PCR
Corresponding TF Gene: GRMZM2G421899
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R16-F 3' Primer Name: R16-R
5' Primer Sequence: caccATGAGCGACCCGCGGGGCCGCGC 3' Primer Sequence: GGATGACCCAAATGGTGCACGCACAAACAGCTG
5' Primer Tm (º C): 75.88 3' Primer Tm (º C): 67.08
PCR Condition:
Nucleotide Sequence: ATGAGCGACCCGCGGGGCCGCGCCGCCGGTCCCGACCGCTACGGGGAGGGCGTACCGCCCGTGGCCGCGACTGTGGAGCC
GGATGGGATTGTTAGCGGCGCAAAGGTGGAGACGAACAGCGAGGACGCCATCAGCCATGACGCCGACGAGGAGGAGAAGG
ACGACGACGATGACTCCACCCAGGATGCGTCTCCGATAGCCGAGGTGAATGTGGAGGGCGAGAGTGGCGCCGTTCACCGC
TTGGAGGTGGAACCGGACCCCTTTCTCGATGGAGATGCCTCTGGGACGGAGGAGGAGCAAGCCGCGTTCATGGTGGAGCT
GGAGCGCTTCCATAAGGAGCACAGTCTCGAGTTCAAGCCGCCCAAGTTCTATGGCAAGGGCCTCAACTGCCTCAAGTTAT
GGAGGCAGGTCGCCCACCTGGGAGGCCACGAGCAGGTAACGGTTTGTAAACTATGGCGTCAAGTTGGAGAGACTTTCAGG
CCGCCAAAGACCTGCACGACTGTTTCTTGGTCATTTCGAATTTTCTATGAGAAGGCACTTCTTGAATACGAAAAACACAA
AGTCCGGACTGGTCAGCTTCAGATACCACCGCCTGGTTTACCACAGCCTAGTGGTGCTGACCGTGAGGTAGTTGTAAATC
AATCATCTTCTGCCAGAGTTAGAAGGGATGTTGCAGCACGTGCTATGCAGGGTTGGCATGCTCACCGCCTCCTTGCCAAT
GGCACTTATGGGGATAACATTTTGAAGGACAAAGATTCAATGCCATCTTCAAGTCGTGATAACCTAAAAAGCTTTGGGGT
GCTCAAGAGAAAGAAAGCATCTAGTCCAGAGTATGCTCTCAAGTCGTCCTGCTCAAAAATAAATAAGTCTGAGGAAGATT
CTATGGTCATTGACGTTGGAGAACCAGCCGACTGGGTGAAGATCAATGTGCGGCAGACTAAAGATTGCTTTGAAGTCTAT
GCACTAGTTCCTGGACTTCTACGTGAGGAGGTGCATGTTCAATCAGATCCTGCTGGACGTCTGGTTATCACAGGGGATCC
AGAGCAGCCTGATAATCCTTGGGAGATCACTCCGTTCAAGAAGGTGGTCAACTTGCCATCAAGAATCAATCCTCACCAAA
CATCCGCTGTTGTCACGCTTCATGGTCAGCTGTTTGTGCGTGCACCATTTGGGTCATCC
TFome clones do not contain stop codon at 3'end.
Translation: MSDPRGRAAGPDRYGEGVPPVAATVEPDGIVSGAKVETNSEDAISHDADEEEKDDDDDSTQDASPIAEVNVEGESGAVHR
LEVEPDPFLDGDASGTEEEQAAFMVELERFHKEHSLEFKPPKFYGKGLNCLKLWRQVAHLGGHEQVTVCKLWRQVGETFR
PPKTCTTVSWSFRIFYEKALLEYEKHKVRTGQLQIPPPGLPQPSGADREVVVNQSSSARVRRDVAARAMQGWHAHRLLAN
GTYGDNILKDKDSMPSSSRDNLKSFGVLKRKKASSPEYALKSSCSKINKSEEDSMVIDVGEPADWVKINVRQTKDCFEVY
ALVPGLLREEVHVQSDPAGRLVITGDPEQPDNPWEITPFKKVVNLPSRINPHQTSAVVTLHGQLFVRAPFGSS
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map