TFome Information for pUT5689

TFome Name:pUT5689
Genbank Insert ID:KJ727748
Protein Name:ZmbHLH17
Species: Maize
TF Family: bHLH
Template: RT-PCR
Corresponding TF Gene: GRMZM2G137358
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R32-F 3' Primer Name: R32-R
5' Primer Sequence: caccATGGAGCCGTCCCCGGCGGTC 3' Primer Sequence: TGAAACCCTTTCATAGCCACTGCCAATCT
5' Primer Tm (º C): 71.2 3' Primer Tm (º C): 61.82
PCR Condition:
Nucleotide Sequence: ATGGAGCCGTCCCCGGCGGTCGCTGCGGAGCTCTGGTGCTCACCGCACCTCGCCGCGGGCGGCGGCCGCCCGTTGGAGGC
GACCTCGGCCGTCACGGAGAAGAGCAGCGGCGGCCGCGGAGGAGGCGGCGCCGTCCGGAGGAGGCCGAGGGAGACCCCGG
TGTCCGAGGATGACTCGTCCCGGATCGTCTCCACTTCCGGCGGTGGCGGCGGCCAGGATTTGACTGATCTAGAAGCAAAG
CGTTCCAAAACAAATAAGTCCAGTAATAATAAAGGCAGTCTAAGAACAGAGGTTGAAACTGACTCAAGAAGTGCTGGCAA
GGCTGTTAGTAAAAACATTCCAGCAGCAGAGCCACCAAAACAAGATTATATCCATGTCAGGGCAAGGAGGGGTCAAGCAA
CTGATAGTCATAGTCTTGCAGAGAGGGCACGTCGTGAGAAAATAAGTGAACGAATGAAAGTTCTCCAAGATCTTGTGCCT
GGATGCAACAAGGTTATTGGCAAAGCATCAGTACTTGATGAGATAATTAATTACATCCAGTCGTTGCAATGTCAAGTTGA
GTTCCTGTCCATGAAACTAGAGGCAGTGAATGCCCAGGCAAACCAGGGAGTTGAAGTATTTCCAGCCAAAGATTACGGTT
CTCAGACATACAACACAGCTCCTGGGCTGACATTTGATACGCAAACCAGTAGAGAATACGCCCAGGGCACGTCGACCTCG
GAATGGCTTCACATGCAGATTGGCAGTGGCTATGAAAGGGTTTCA
TFome clones do not contain stop codon at 3'end.
Translation: MEPSPAVAAELWCSPHLAAGGGRPLEATSAVTEKSSGGRGGGGAVRRRPRETPVSEDDSSRIVSTSGGGGGQDLTDLEAK
RSKTNKSSNNKGSLRTEVETDSRSAGKAVSKNIPAAEPPKQDYIHVRARRGQATDSHSLAERARREKISERMKVLQDLVP
GCNKVIGKASVLDEIINYIQSLQCQVEFLSMKLEAVNAQANQGVEVFPAKDYGSQTYNTAPGLTFDTQTSREYAQGTSTS
EWLHMQIGSGYERVS
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map