TFome Information for pUT5691

TFome Name:pUT5691
Genbank Insert ID:KJ727750
Protein Name:ZmbHLH22
Species: Maize
TF Family: bHLH
Template: RT-PCR
Corresponding TF Gene: GRMZM2G162450
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R34-F 3' Primer Name: R34-R
5' Primer Sequence: caccATGGACAAAGAGCAGCAGGAGAGTC 3' Primer Sequence: CAGGCTGTAGTTGACGTCGATGAAG
5' Primer Tm (º C): 63.79 3' Primer Tm (º C): 59.23
PCR Condition:
Nucleotide Sequence: ATGGACAAAGAGCAGCAGGAGAGTCACCTCGACAGCTTCGCGCCGCTGGACGGCGCCGCGGCGCCGGAGGACCAGCAGCA
CCAGCCCGCCTGCGGCGGGGGGCCCGAGATGGTCGACTACATGCTTGGCCGCCACCACGTGAACGCGCACGCGCCGCCGT
CGCCGACGCAGAGCCAAGCGGTGTCGTTCGACAAGCTCAGCTTCTCCGACGTGCTGCAGTTTGCCGACTTCGGCCCCAAG
CTCGCGCTCAACCATCCGGTGGCCGCGTCCGTCGGCGGCGGCGAGGACGCGGACGCGGACGACGACGACGACGGGTACTT
CTTCAGGTTCCAGTCGCTGCCCCAGCGCCATGCGGACCGTGAGGCGGCGGGAAGCAAGACGACGGCCGAGGACGGTGGAG
GAACCGGCGTCGTCGTCTCCGAGGGCACCACGCTGGTGCAGCAGCAGGCCGATCACGGCCGGGCGGACAAAGCCGGGGAC
CAGGGGAAGAGCGGCCGCCGGAAGCGCCCGCGGACCGTGAAGACCAGCGAGGAGGTGGAGAGCCAGCGGATGACGCACAT
CGCCGTCGAGCGCAACCGCCGGCGCCAGATGAACGAGTACCTCAGGATCCTCAGGTCACTCATGCCAGGATCCTACGTCC
AGAGGGGAGACCAGGCATCCATCATAGGAGGCGCCATCGAGTTCATAAGAGAGCTAGAGCAGCTGATCCAGTGCCTGGAG
TCGCAGAAGCGGCGGCGCCTGTACGGCGGGTCCGGGGACGCGCCACGGCCGCCGGTGGTGGACGCGGCAGCGGGCTCCGG
CGGCGCGCTAATAACGTCCTCCACTCAGCCGCTGGCGCTGCAGCCGCCGCACCTGTTCCCTCCGACCCCGAGCCACCCTT
TCCCGGTGGCGGGCGCCGACGCCAAGATCACGCTGGACCTGGAGGCGGCGGGGGGCGCCGTGGTCGACGACGCCGGCGGC
GGGCTCCGGGAGGAGGTGGCGGAGAACAAGTCGTGCCTGGCGGACATCGAGGTGCGCGCGCTGGGCGCCGATGCCATGAT
CAAGATCCTATCCCGGCGCCGGCCAGGCCAGCTGATCAAGACCATCGCCGCCCTGGAGGACATGCAGATGTCCATCCTGC
ACACCAACATCACCACCATCGAGCAGACCGTCCTCTACTCCTTCAACGTAAAAATCGTCGGCGAGGCAAGGTACTCGGCG
GAGGACATCGCCGGCGCCGTGCACCAGATCCTCAGCTTCATCGACGTCAACTACAGCCTG
TFome clones do not contain stop codon at 3'end.
Translation: MDKEQQESHLDSFAPLDGAAAPEDQQHQPACGGGPEMVDYMLGRHHVNAHAPPSPTQSQAVSFDKLSFSDVLQFADFGPK
LALNHPVAASVGGGEDADADDDDDGYFFRFQSLPQRHADREAAGSKTTAEDGGGTGVVVSEGTTLVQQQADHGRADKAGD
QGKSGRRKRPRTVKTSEEVESQRMTHIAVERNRRRQMNEYLRILRSLMPGSYVQRGDQASIIGGAIEFIRELEQLIQCLE
SQKRRRLYGGSGDAPRPPVVDAAAGSGGALITSSTQPLALQPPHLFPPTPSHPFPVAGADAKITLDLEAAGGAVVDDAGG
GLREEVAENKSCLADIEVRALGADAMIKILSRRRPGQLIKTIAALEDMQMSILHTNITTIEQTVLYSFNVKIVGEARYSA
EDIAGAVHQILSFIDVNYSL
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map