TFome Information for pUT5693

TFome Name:pUT5693
Genbank Insert ID:KJ727752
Protein Name:ZmbHLH175
Species: Maize
TF Family: bHLH
Template: RT-PCR
Corresponding TF Gene: GRMZM2G173534
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R37-F 3' Primer Name: R37-R
5' Primer Sequence: CCACATGGACGACTCGGCGGAGGTGAAGCTGGTG 3' Primer Sequence: CATTGCGTTGTGGAGGCCGGCGGTGTG
5' Primer Tm (º C): 70.59 3' Primer Tm (º C): 69.24
PCR Condition:
Nucleotide Sequence: ATGGACGACTCGGCGGAGGTGAAGCTGGTGGACGAGATCACGGGAGAGGGAGGGGCGGCGGGGGACTGGGGCTACCTGGG
CTCCGACGGGATGGGCTCCGGCTCCTACCCGGCGTTCCCCTTCTCCAGAGACGTCCTATCCACGCCGACCTCCGCGTCGT
TGCTCCTCTCCATGGACCCCGCGGCGCTCTTCGACTTCAACGGCACCTTCCCTCCCTCCTCCGCGGCCGCGGCCACCGCC
GGCTCCTCGCTGTCCGCCTTCCACGACTTCAGCTGCATCAACCCCTTCGACGACGCCGGCCACTTCCTCGGCGCTCCCCC
GCCGGTGCCGGCGGCGGCGGCGCCTCAGCAGCAGGGGCAGAAAGGTGGGTTCTTTGCGCCGCTGCCGGCCTCGGACTTCA
ACGACGCCGGGATGAGCTGGGACGACGAGGACGAGATCGACCAAAGCGTCGACGCCTCCTCGATGGCCATCTCGGCCTCC
ATGGAGAATGCTGCGGGCGCGGTGGCGGGGGCAAGTGGCGCCGGCGGTGGCAGCGGGAGAGGCAAGAAGAAAGGGATGCC
GGCCAAGAACCTCATGGCGGAGCGCCGGCGTCGCAAGAAGCTCAATGACCGCCTCTACATGCTGCGCTCGGTGGTGCCGA
AGATCAGCAAGATGGATAGGGCTTCTATCCTTGGCGACGCAATTGAGTATCTGAAGGAGCTCCTGCAGAGGATCAGCGAT
CTTCACAATGAACTTGAGTCAGCTCCGAGCTCTTCGCTTGTTGGACCAACATCAGCTAGCTTCAACCCATCAACACCGAC
ATTGCAGACATTTCCTGGCCAGGTCAAGGAAGAACTTTGCCCGGGCTCATTCCCAAGCCCTACTGGACAACAAGCCACAG
TCGAAGTCAGGATGAGGGAAGGGCATGCAGTCAATATCCACATGTTCTGTGCGCGTAGGCCAGGCATCCTGCTGTCGACA
ATGACGGCCCTCGACAGCCTAGGCCTCGACATCGAGCAGGCGGTCATCAGCTGCTTCAATGGCTTTGCGATGGACGTGTT
CCGAGCTGAGTGCGCGGATGGTCCTGGAATGGTGCCTGAAGAAATTAAGGCCGTGCTGATGCACACCGCCGGCCTCCACA
ACGCAATG
TFome clones do not contain stop codon at 3'end.
Translation: MDDSAEVKLVDEITGEGGAAGDWGYLGSDGMGSGSYPAFPFSRDVLSTPTSASLLLSMDPAALFDFNGTFPPSSAAAATA
GSSLSAFHDFSCINPFDDAGHFLGAPPPVPAAAAPQQQGQKGGFFAPLPASDFNDAGMSWDDEDEIDQSVDASSMAISAS
MENAAGAVAGASGAGGGSGRGKKKGMPAKNLMAERRRRKKLNDRLYMLRSVVPKISKMDRASILGDAIEYLKELLQRISD
LHNELESAPSSSLVGPTSASFNPSTPTLQTFPGQVKEELCPGSFPSPTGQQATVEVRMREGHAVNIHMFCARRPGILLST
MTALDSLGLDIEQAVISCFNGFAMDVFRAECADGPGMVPEEIKAVLMHTAGLHNAM
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map