TFome Information for pUT5754

TFome Name:pUT5754
Genbank Insert ID:KJ727802
Protein Name:ZmALF18
Species: Maize
TF Family: Alfin-like
Template: BT036116.1
Corresponding TF Gene: GRMZM2G008259
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: B405_F 3' Primer Name: B405_R
5' Primer Sequence: caccATGGATATGGCCCCCGC 3' Primer Sequence: CTGCCTTATTTTCTTCGAGCTGCA
5' Primer Tm (º C): 68.4 3' Primer Tm (º C): 64.7
PCR Condition: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGACGGCGGCGGCACGCACCGCACGCCGGAAGACGTGTTCCGGGATTTCCGCGCGCGGCGGGCTGGTATGATCAAAGC
GCTCACCACCGACGTGGAGAAGTTCTACCAGCAGTGTGACCCAGAGAAAGAGAATCTGTGTTTGTATGGTCTTCCCAATG
AAACATGGGAAGTGAACTTGCCTGCAGAGGAGGTTCCTCCTGAACTTCCAGAGCCAGCTCTGGGAATTAATTTTGCTCGT
GACGGGATGGATGAAAAAGATTGGCTGTCACTAGTCGCGGTGCACAGTGATGCTTGGTTGCTGGCTGTGGCCTTTTACTT
CGGTGCAAGATTTGGTTTTGACAAAGAATCCAGGAAGCGCCTTTTTGTCATGATTAATAACCTCCCTACAATATATGAAG
TTGTCACGGGAACTGCCAAGAAGCAAACCAAGGAGAAAACTCCGAAAAGCAGCAGCAAGAGCAATAAAGCTGGCCCAAAA
CCGCCACGCCAGCCAGAACCCAACTCAAGGGGTTCGAAGATGCCACCTCCGAAGGATGAGGATGACAGCGGAGGCGAGGA
AGAGGAGGAAGAGGAAGATCACGAAAACACGCTGTGTGGTTCTTGTGGTGACAACTACGGACAGGATGAGTTCTGGATAT
GCTGCGATGCGTGCGAGACTTGGTTCCATGGCAAGTGTGTCAAGATCACTCCTGCCAAGGCCGAACACATCAAGCACTAC
AAGTGTCCGAACTGCAGCGGTAGTGGCAAGAGAGCCCGAGCA
TFome clones do not contain stop codon at 3'end.
Translation: MDGGGTHRTPEDVFRDFRARRAGMIKALTTDVEKFYQQCDPEKENLCLYGLPNETWEVNLPAEEVPPELPEPALGINFAR
DGMDEKDWLSLVAVHSDAWLLAVAFYFGARFGFDKESRKRLFVMINNLPTIYEVVTGTAKKQTKEKTPKSSSKSNKAGPK
PPRQPEPNSRGSKMPPPKDEDDSGGEEEEEEEDHENTLCGSCGDNYGQDEFWICCDACETWFHGKCVKITPAKAEHIKHY
KCPNCSGSGKRARA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map