TFome Information for pUT5755

TFome Name:pUT5755
Genbank Insert ID:KJ727803
Protein Name:ZmALF10
Species: Maize
TF Family: Alfin-like
Template: BT086399.1
Corresponding TF Gene: GRMZM2G050495
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B409_F 3' Primer Name: B409_R
5' Primer Sequence: caccATGGCGGACGCCGCCTCT 3' Primer Sequence: CTGCCTCGCCCTCTTGGAGCTGCAG
5' Primer Tm (º C): 73.7 3' Primer Tm (º C): 72.7
PCR Condition: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGACAGGCACGGCTCGGTTCGGCTGCCCACCCCCGAGGATGTCTTCACGGATTTCCGCGCCCGCCGTGACGGCATTCT
CAAGGCACTCACCACCGATGTGGAGGAGTTCTACAAGTTGTGCGACCCAGAGTATGAGACCCTGTGCCTGTATGGACTAC
CTAACGAAACCTGGAAAGTGAACGTTCGTCCTGCGAAAGTTCCTCAACAACTACCAGAGCCAGCAGAGGGAATAAACATT
CCTCGCGATTCAATGCCAAAGGAAGATTGGCTCTCGTTCGTAGCTGCACACAGTGATGCATGGTTGGTTGCTATGGCATT
TCACTTTGGGGCTCTCTTCGGATTTGATAAAGACGCCAGGAGACGGCTCCACATGATGATCAATAATCATCCCACTGTAT
CTGAAGTTGTGATAGGGAGTGGGGAGAAGCAACCAAAAGCCTGCAACACAAATTACGAAACCAAGTCAAGTTCTATTAAG
GAACCCAGTTCTAGCAGCAAGCTAGCAGAGGAGCCTTTGCCAAAGAAGGAGCGACAGATCATCAAAGAAGATGGAGGCGG
CGAGGATGAAGCTTACCCATGCGGCACATGTGGAGGGATGTACTCGGAAAATGGAGTATTCTGGATTGGCTGTGACATTT
GCGACAAGTGGTACCATGGTGACTGTGTTCGCATCACACCTGCGGAGGCAACGCACATCGATCAGTACAGTTGCCCCGCT
TGCAGCAACAAGAGGAGCAGAGAG
TFome clones do not contain stop codon at 3'end.
Translation: MDRHGSVRLPTPEDVFTDFRARRDGILKALTTDVEEFYKLCDPEYETLCLYGLPNETWKVNVRPAKVPQQLPEPAEGINI
PRDSMPKEDWLSFVAAHSDAWLVAMAFHFGALFGFDKDARRRLHMMINNHPTVSEVVIGSGEKQPKACNTNYETKSSSIK
EPSSSSKLAEEPLPKKERQIIKEDGGGEDEAYPCGTCGGMYSENGVFWIGCDICDKWYHGDCVRITPAEATHIDQYSCPA
CSNKRSRE
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map