TFome Information for pUT5756

TFome Name:pUT5756
Genbank Insert ID:KJ727804
Protein Name:ZmbZIP38
Species: Maize
TF Family: bZIP
Template: BT070122.1
Corresponding TF Gene: GRMZM2G073892
Transcript: T01
Vector: pENTR/SD/D-TOPO
Note: c.A455G p.Q152R Present in template FLcDNA, present in functional domain.

5' Primer Name: C276_F 3' Primer Name: C276_R
5' Primer Sequence: caccATGCCGAGGAGCCCGCA 3' Primer Sequence: GAGCTGAGTGGATGAGGAGGTTCTTCTGA
5' Primer Tm (º C): 72.1 3' Primer Tm (º C): 69
PCR Condition: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 40 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGCCGAGGAGCCCGCAGCTGAGCCTGAGCGGCTGCAGCTCTCTCTTCTCCCTGTCCAGCACCAGTACGAGCCGCGACAG
CGCCGCCCTGAAGGCGCCGGTGCCAGCGCCACCGGCAGGCGCGGTGCCCCCACGGCGCACCCCTCTCCTGTCGCTCAGCG
TCGGCGAGGAGGAAGAGGACGAGGGTGAGGACGAGGAGGAGTACCTGCTGGGGGCCGGCGCCCTGGATCTGCAGCTGACG
GGGCCCGGTGGCGGCAGCAACAGCAGCGGCGGCTACGACGACGAGCGGAAGAACATCCGGATGATGAGGAACAGGGAGTC
TGCGCTGCGCTCCAGGGCAAGGAAGAGGGCCTATGTGCAGGAGCTGGAGAAGGAGGTTCGTCGCCTGGTGAATGAGAACC
TCAAGCTCAAGAGGCAGTGCAAACAACTGAAACTAGATATGGCTGCACTGGTCCGGCAAAGCAGCAGCAAGAGCAGCTCC
CACATCAGAAGAACCTCCTCATCCACTCAGCTC
TFome clones do not contain stop codon at 3'end.
Translation: MPRSPQLSLSGCSSLFSLSSTSTSRDSAALKAPVPAPPAGAVPPRRTPLLSLSVGEEEEDEGEDEEEYLLGAGALDLQLT
GPGGGSNSSGGYDDERKNIRMMRNRESALRSRARKRAYVQELEKEVRRLVNENLKLKRQCKQLKLDMAALVRQSSSKSSS
HIRRTSSSTQL
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map