TFome Information for pUT5764

TFome Name:pUT5764
Genbank Insert ID:KJ727812
Protein Name:ZmbZIP86
Species: Maize
TF Family: bZIP
Template: BT056226
Corresponding TF Gene: GRMZM2G171370
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B826_F 3' Primer Name: B826_R
5' Primer Sequence: caccATGGACATTTGGAATACTTCTCCTG 3' Primer Sequence: CCTAGTGTTAGCCTCCGGTTTGTGG
5' Primer Tm (º C): 59.1 3' Primer Tm (º C): 61.1
PCR Condition: 5 l Phusion GC buffer 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (Thermo Fischer) 10.75 l ultrapure water 40 sec 98°C 30x : 15 sec 98°C 30 sec 65°C (prior 58°C) 40 sec 72°C 7 min 72°C leave at 10°C
Nucleotide Sequence: ATGGACATTTGGAATACTTCTCCTGCCTTGGCTGTTCCTGCAGTGCAGGGGGAAGCGAATCCTGGGTTGGCGCTTGCCCG
ACGTGATAGCGTTACTCAACTGGATGAACGTGAATTGAAGAGGGAGAGGCGGAAACAATCTAACAGGGAGTCTGCAAGGA
GATCTAGGTTACGCAAGCAGCAAGAGTGTGAGGAATTAGCCAGGAAGGTAGCTGACCTAACAACTGAGAATAGTGCTCTC
AGAGCGGAACTTGACAACCTGAGGAAGGCTTGCCAAGACATGGAAGCAGAAAATTCACGTCTATTGGTGAGCACGGTACC
AAGTGTCACAACCACACTGGGAATGAGCATCGAGCCGCCAAAGGCGCAGCAGCATCATGACGACGAGGGCCAGCTCCACA
AGAAATCTAGTAATAACAGCAACGGGAAAGGAAGCCACAAACCGGAGGCTAACACTAGG
TFome clones do not contain stop codon at 3'end.
Translation: MDIWNTSPALAVPAVQGEANPGLALARRDSVTQLDERELKRERRKQSNRESARRSRLRKQQECEELARKVADLTTENSAL
RAELDNLRKACQDMEAENSRLLVSTVPSVTTTLGMSIEPPKAQQHHDDEGQLHKKSSNNSNGKGSHKPEANTR
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map