TFome Information for pUT5885

TFome Name:pUT5885
Genbank Insert ID:KJ727841
Protein Name:ZmCOL13
Species: Maize
TF Family: C2C2-CO-like
Template: RT-PCR
Corresponding TF Gene: GRMZM2G038783
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R170F 3' Primer Name: R170R
5' Primer Sequence: caccATGGAGCTGCACAAGTACTGGGG 3' Primer Sequence: AAAGCTCGGTACGACGCCGTAG
5' Primer Tm (º C): 64.7 3' Primer Tm (º C): 61.2
PCR Condition:
Nucleotide Sequence: ATGGAGCTGCACAAGTACTGGGGCGTGGGGGGCCGGCGGTGCGGCAGCTGCGAGGGGGCCCCGGCGGCGGTGCACTGCCG
CACGTGCGTTGGTGGATCCTTCCTCTGCACGACGTGCGACGCCCGGCCCGCGCACGCGCGCCTGGGCCACGAGCGCGTGT
GGATGTGCGAGGTCTGCGAGCTGGCCCCCGCCGCCGTCACGTGCAAGGCCGACGCCGCCGTGCTCTGCGCCGCCTGCGAC
TCCGACATCCACGACGCCAACCCGCTGGCCCGGCGCCACGCGCGGGTCCCCGTCGCCCCCATCGGCTCCGAGGCCGCCGC
CGCGGCCGTGGAGGCCATGCTGTTCGGGACCGGCGAGGCGGCCGCTTCCGAGGCCGACGAGCAGCACGCGGCGGCCGAGC
ACGCGCACGCGCACGCGCACGCGCTGAACCTCAACGTGGAGGCCAAGGACATGAAGCTGGACTACCTCTTCTCTGAACTG
GATCCCTACCTCAGCGTCGAGATCCCGCGCTTCCAGCACGCTGACAGCGTCGTCCCCAACGGCGCCGGCGCTGCCGTCGA
GCTGGACTTCACGTGCGGCATCGGCGTTAAGCACTCCTCCTACAGCTCCTACACGGCCACCTCCCTCGCTCATAGCGGCT
CCTCGTCGGAGGTTGGCGTGGTGCCAGAGGCCTTCGGCGGCAGCGGCAGCGGCGGCGGGAGCTTTGAGCTCGACTTCACA
AGGCCCAAGCCTCAGGCCTACATGCCATACACCGGGACTCCCCAGAGCCACAGCGTGCCATCCGCGGACGTGGAGGTGGT
GCCGGAGCGGGGAGACTTGGCTGCGGTGAGGCCGGTGCCGCTGATGGGGGAGAGCAGGGAGGCGCGCCTGATGCGGTACC
GTGAGAAAAGGAAGAACAGGCGCTTCGAGAAGACCATCCGGTACGCGTCCCGGAAGGCCTACGCCGAGACGCGGCCGCGA
ATCAAGGGCCGGTTTGCCAAGCGTGCTGACCACGACGGGGACGCCGACGCCGACGACGCTGAGGCCGAGGCCGAGGCCGC
CGTGCCGATGTCATATGTGCTCGACTTCGGCTACGGCGTCGTACCGAGCTTT
TFome clones do not contain stop codon at 3'end.
Translation: MELHKYWGVGGRRCGSCEGAPAAVHCRTCVGGSFLCTTCDARPAHARLGHERVWMCEVCELAPAAVTCKADAAVLCAACD
SDIHDANPLARRHARVPVAPIGSEAAAAAVEAMLFGTGEAAASEADEQHAAAEHAHAHAHALNLNVEAKDMKLDYLFSEL
DPYLSVEIPRFQHADSVVPNGAGAAVELDFTCGIGVKHSSYSSYTATSLAHSGSSSEVGVVPEAFGGSGSGGGSFELDFT
RPKPQAYMPYTGTPQSHSVPSADVEVVPERGDLAAVRPVPLMGESREARLMRYREKRKNRRFEKTIRYASRKAYAETRPR
IKGRFAKRADHDGDADADDAEAEAEAAVPMSYVLDFGYGVVPSF
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map