TFome Information for pUT5890

TFome Name:pUT5890
Genbank Insert ID:KJ727846
Protein Name:ZmABI28
Species: Maize
TF Family: ABI3-VP1
Template: RT-PCR
Corresponding TF Gene: GRMZM2G109879
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R84F 3' Primer Name: R84R
5' Primer Sequence: caccATGCCATTGCCCAGCGACTCT 3' Primer Sequence: GCAACGGCGTGTGATGACGA
5' Primer Tm (º C): 65.5 3' Primer Tm (º C): 60.8
PCR Condition:
Nucleotide Sequence: ATGCCATTGCCCAGCGACTCTGGTTCTGGTGCGCGCGCCATGTCGAAGCAGTTCAAGGTCCTGCTTCCTACTTCCTTCCA
CAAGCTTCGCATCTCCGACGAGCTCGCCGGTTGCTTCGACGCCGGCGAGGGCGAAGGGGCGCCCGAGCCGACGGCCCTCG
TGGTCAGTCCGTTCGGGAAGGTGTGGCGTGTGGAGGTGGTCCGGGACGGCCACGGTGCGTCGCTCGGGCGCGGGTGGGCG
GAGTTCTTGGCCGCGCACGGCGTCGGCATCGGCTGGTTCGTCGTGCTCCGTTATGAGGGCGGCGGCGCGCTCACCGTCAA
GGTGTTCGACACCAGCTTGTGCATTAAGGAGTTCGGCGCTCCGGCTGCAGTTATGCCATCCAAAAGCTGCAAAAGTATTT
TCTGTAAGCCACAATTCATCAGGATTTTTCATTCGAATCTCTCGGAAAAGATGATGTTGCCTGCTAGGTTTGTGAAAGAT
TACGTCAGTGAAGAGTGCCTAAACAGCCGGATAGCCATCATTTTCAGCCCCATTGCCAAATTTTGGCGTGTTGAGCTTAA
GAACGATCAATCAGGCATCTTCTTCACAGGTGGCTGGTCACAGTTTCTAGACTTCCATGGAATAAAGAATGGTGAAGTTT
TGCTCTTGAAATACGAAGGGAACATGGTTTTCAAATTCAAAGCATTTGGACTTAGTGGATGCCAGAAAGATTTCAAGAAC
CAGAATGCTGGGATTCAACTAAATACTAAAAAACAACAGGAAACACCTCCCATCAGGAAACGTAAAAGCAATGATGAGAA
GTCAAGCAGTGAAGAAAACAAGAGGCTAAAAACTCCAGTGACTTCTCCATCACCTTCTGATCCGTCACTGGAAAAACCCT
ATCAGATTGGGACATCATCTTGGATAAAGAAAAGGATCAACACCTATGCACTTGAGCAATTTCTTGCGTTATCAGTGAAG
TTCTGCAACTCGATTGGGTTCCGGATGGCTTGCACAATCACCCTTAAGACAGCAACGAACAGCACCAGATCATGGCAGGT
GCGTGGTACTGCATACAAGTGGCATCGCTACATCATCGGAGTGGGCTGGAAGAGCTTCTGCCAGGATAACAGACTCAAGG
CAGGCGACCTGTGCACCTTCAACATCATCGAGACCACGCTGTGGCATGTTACTATCACGCGCTCAGCATTAGCAGCAGAT
ACTTTTAATAAGCAAAAAAAGCCACCTTGTTCATCCAGTAGGGACAAGAAGGGCAGTTCAAGCAGAGAAGGAGCAAAGAG
GCCGAAACCTTCCATGGCACATTTCAGCAAGGTTCAGGTTCCGCCATCGTACGCAAGACGATGTGCCTACGACGTTGGAC
CGCCGCCCTGGATACGGAAGCAGATGAATGCCAGCACAATAAGAAATCGTCTTTGTCTGGCACCGGCCTTCTGCAATCAG
ATTGAGTTGCGGCGACGCTGCGAGATCGTGCTCAAGACCTCGATCAACAGCAATCATTCTTGGCGGGTTGGTGGTCTCTC
GCAGAAGAAGGGTAGTTACATTATTGGGCCCGGTTGGAAAAAATTCTGCACGGAGAACAGAGTTAAGGTTGGTGATGTCT
GCACCTTCAATGTCATCGAAACCCAGCTGTGGCATGTCGTCATCACACGCCGTTGC
TFome clones do not contain stop codon at 3'end.
Translation: MPLPSDSGSGARAMSKQFKVLLPTSFHKLRISDELAGCFDAGEGEGAPEPTALVVSPFGKVWRVEVVRDGHGASLGRGWA
EFLAAHGVGIGWFVVLRYEGGGALTVKVFDTSLCIKEFGAPAAVMPSKSCKSIFCKPQFIRIFHSNLSEKMMLPARFVKD
YVSEECLNSRIAIIFSPIAKFWRVELKNDQSGIFFTGGWSQFLDFHGIKNGEVLLLKYEGNMVFKFKAFGLSGCQKDFKN
QNAGIQLNTKKQQETPPIRKRKSNDEKSSSEENKRLKTPVTSPSPSDPSLEKPYQIGTSSWIKKRINTYALEQFLALSVK
FCNSIGFRMACTITLKTATNSTRSWQVRGTAYKWHRYIIGVGWKSFCQDNRLKAGDLCTFNIIETTLWHVTITRSALAAD
TFNKQKKPPCSSSRDKKGSSSREGAKRPKPSMAHFSKVQVPPSYARRCAYDVGPPPWIRKQMNASTIRNRLCLAPAFCNQ
IELRRRCEIVLKTSINSNHSWRVGGLSQKKGSYIIGPGWKKFCTENRVKVGDVCTFNVIETQLWHVVITRRC
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map