TFome Information for pUT5895

TFome Name:pUT5895
Genbank Insert ID:KJ727850
Protein Name:ZmCOL6
Species: Maize
TF Family: C2C2-CO-like
Template: RT-PCR
Corresponding TF Gene: GRMZM2G013398
Transcript: T03
Vector: pENTR/SD/D-TOPO

5' Primer Name: R168F 3' Primer Name: R168R
5' Primer Sequence: caccATGGGCGCTCTCTGTGATTTCTGT 3' Primer Sequence: GTAACTCCTCGTTTGGCAGAGAGGATCA
5' Primer Tm (º C): 64 3' Primer Tm (º C): 61.3
PCR Condition:
Nucleotide Sequence: ATGGGCGCTCTCTGTGATTTCTGTGGGGAGCATAGGTCTATGGTTTACTGCCGATCAGATGCAGCATCCTTGTGTTTATC
ATGTGACCGTAATGTGCATTCAGCTAATGCTCTTTCTCGGCGTCATACAAGGACACTTTTATGTGACCGGTGTGCTTCAC
AGCCTGCTATGGTCCGCTGTCTAGCAGAAAATGCCTCGCTTTGCCAAAATTGTGATTGGAATGGCCACATTGCAGGATCC
TCATCTGCCGGACACAAAAGGCAGACTATAAATTGCTATTCAGGATGTCCATCGTCTGCAGAACTTTCTAGAATATGGTC
CTTCGTTTCAGATATTCCTAATGTAGCACCTGAGCCAAACTGTGAGCAAGGAATAAGCATGATGAGCATCAGCGACAGTG
GTGTCAGTAATCAAGATAATGCTGCAGGGGATAGTATTTTGTTAGATATAGCTAGTGCAACACTTGTAAGTGATATTGGT
ACTTGTGACAAGCTTTTGGTAGGTTCCTCTTCAGGAGCTGGAGTGAACCTACTTCCACTGGCTACTGGTCAAACAGAAAC
AGCTGGATCTGTGGATTCCACACCAGATAAGGTTCCCTATACGCCAGATAAAGATATGTTCAGTAAGGACAGCATATATG
AAGATTTTTGTGTGGATGACGCTGACCTTGCTTTCGAAAATTATGAAGAACTCTTTGGTACCTCTCATATTCAAACAGAA
CAACTCTTTGATGATGCTGGAATAGACATTTACTTTGAAATGAAGGAAGCACCTGCTGGGAATTCCACTGAGTCCAAGCT
GAAGCAACCTGCAAATAGCAATGCGGTATCTGCCGACTCTGGGATGTCGAACCCTGGAGTGAAAGGTGATTCCAGTGTTT
GTACCCCTTTGAGGCAGGCTAGGTCTAGTCTGTCTCTTTCCTTTGCTGGTTTGACTGGTGAGAGTAGTGCGGGAGACCAC
CAAGATTGTGTGGTATCATCATTGCTTCTTATGGGTGAGCCTCCTTGGCAGCCTCCTGGCCCCGAGGGCTCAATTGCTGG
AGGTAGTAGAGACAGTGCACTCACACGATACAAGGAGAAGAAAATGAGAAGAAAGTTCGACAAGAAAATCAGGTACGCTT
CTCGTAAGGCTAGAGCAGATGTGCGAAAGAGGGTAAAGGGTCGGTTTGTTAAGGCTGGTGAAGCATATGACTATGATCCT
CTCTGCCAAACGAGGAGTTAC
TFome clones do not contain stop codon at 3'end.
Translation: MGALCDFCGEHRSMVYCRSDAASLCLSCDRNVHSANALSRRHTRTLLCDRCASQPAMVRCLAENASLCQNCDWNGHIAGS
SSAGHKRQTINCYSGCPSSAELSRIWSFVSDIPNVAPEPNCEQGISMMSISDSGVSNQDNAAGDSILLDIASATLVSDIG
TCDKLLVGSSSGAGVNLLPLATGQTETAGSVDSTPDKVPYTPDKDMFSKDSIYEDFCVDDADLAFENYEELFGTSHIQTE
QLFDDAGIDIYFEMKEAPAGNSTESKLKQPANSNAVSADSGMSNPGVKGDSSVCTPLRQARSSLSLSFAGLTGESSAGDH
QDCVVSSLLLMGEPPWQPPGPEGSIAGGSRDSALTRYKEKKMRRKFDKKIRYASRKARADVRKRVKGRFVKAGEAYDYDP
LCQTRSY
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map