TFome Information for pUT5958

TFome Name:pUT5958
Genbank Insert ID:KJ727866
Protein Name:ZmbZIP1
Species: Maize
TF Family: bZIP
Template: BT036023.1
Corresponding TF Gene: GRMZM2G015534
Transcript: T01
Vector: pENTR/SD/D-TOPO
Note: c.G421A p.E141K; c.A1002T p.S334(=). SNPs present in template FlcDNA. Not in functional domain.

5' Primer Name: B473_F 3' Primer Name: B473_R
5' Primer Sequence: caccATGCCGCCGACGACCCA 3' Primer Sequence: ATACATGTCCATGTGTATGGCCCCATTTG
5' Primer Tm (º C): 72.1 3' Primer Tm (º C): 67.9
PCR Condition: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGCCGCCGACGACCCATCATATCTATGGTTACTTATTATTGGGCATGGAGCACGTCATCTCAATGGAGGAGATCCTCGG
GCCCTTCTGGGAGCTGCTACCACCGCCAGCGCCAGAGCCAGAGCCAGAGCGAGAGCAGCCTCCGGTAACCGGCATCGTCG
TCGGCAGTGTCATAGACGTTGCTGCTGCTGGTCACGGTCATGGTGGCGGCGACATGATGGATCAGCAGCACGCCACAGAG
TGGACCTTTGAGAGGTTACTGGAAGAGGAGGCTCTGACGACAAGCACACCGCCGCCGGTGGTGGTGGTGCCGAACTCTTG
TTGCTCAGGCGCCCTAAATGTTGACCGGCCGCCGGTGATGGAAGAGGCGGTAATGATGGCGCCTGCGGCGGTGAGTAGTG
CCGTAGTAGGGGACCCCATGAAGTACAATGCCATACTGAGGAGGAAGCTGGAGGAGGACCTCGAGGCCTTCAAAATGTGG
AGGGCGGCCTCCAGTGTTGTGACCTCAGATCAACGTTCTCAAGGCTCAAACAATCACACTGGAGGTAGCAGCATCAGGAA
TAATCCAGTGCAGAACAAGCTGATGAACGGCGAAGATCCAATCAACAATAACCACGCTCAAACCGCAGGCCTTGGCGTGA
GGCTTGCTACTAGCTCTTCCTCGAGAGATCCTTCACCATCAGACGAAGACATGGACGGAGAAGTAGAGATTCTGGGGTTC
AAGATGCCTACCGAGGAAAGAGTGAGGAAAAGAAAGGAATCCAATAGAGAATCAGCCAGACGCTCGAGATACAGGAAAGC
CGCTCACCTGAAAGAACTGGAGGACCAGGTAGCACAGCTAAAAGCCGAGAATTCTTGCCTGCTGAGGCGCATTGCCGCTC
TGAACCAGAAGTACAACGACGCTAACGTCGACAACAGGGTGCTGAGAGCGGACATGGAGACCCTAAGAGCTAAGGTGAAG
ATGGGAGAGGACTCCCTGAAGCGGGTGATAGAGATGAGCTCTTCAGTGCCGTCGTCCATGCCCATCTCGGCGCCGACCCC
CAGCTCCGACGCTCCAGTGCCGCCGCCGCCTATCCGAGACAGCATCGTCGGCTACTTCTCCGCCACAGCCGCAGACGACG
ATGCTTCGGTCGGCAACGGTTTCTTGCGACTGCAAGCTCATCAAGAGCCTGCATCCATGGTCGTCGGTGGAACTCTGAGC
GCCACAGAGATGAACCGAGTAGCAGCAGCCACGCATTGCGCGGGGGCCATGGAGCTCATCCAGACGGCGATGGGATCCAT
GCCGCCGACCTCCGCCTCCGGATCTACACCGCCGCCGCAGGATTATGAGCTGCTGGGTCCAAATGGGGCCATACACATGG
ACATGTAT
TFome clones do not contain stop codon at 3'end.
Translation: MPPTTHHIYGYLLLGMEHVISMEEILGPFWELLPPPAPEPEPEREQPPVTGIVVGSVIDVAAAGHGHGGGDMMDQQHATE
WTFERLLEEEALTTSTPPPVVVVPNSCCSGALNVDRPPVMEEAVMMAPAAVSSAVVGDPMKYNAILRRKLEEDLEAFKMW
RAASSVVTSDQRSQGSNNHTGGSSIRNNPVQNKLMNGEDPINNNHAQTAGLGVRLATSSSSRDPSPSDEDMDGEVEILGF
KMPTEERVRKRKESNRESARRSRYRKAAHLKELEDQVAQLKAENSCLLRRIAALNQKYNDANVDNRVLRADMETLRAKVK
MGEDSLKRVIEMSSSVPSSMPISAPTPSSDAPVPPPPIRDSIVGYFSATAADDDASVGNGFLRLQAHQEPASMVVGGTLS
ATEMNRVAAATHCAGAMELIQTAMGSMPPTSASGSTPPPQDYELLGPNGAIHMDMY
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis:
 
Plasmid Map