TFome Information for pUT5977

TFome Name:pUT5977
Genbank Insert ID:KJ727884
Protein Name:ZmABI33
Species: Maize
TF Family: ABI3-VP1
Template: gDNA
Corresponding TF Gene: GRMZM2G065538
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: P03up 3' Primer Name: P03dn
5' Primer Sequence: caccATGCTGCGCAAGCACACGTACT 3' Primer Sequence: AGAGAAAGATCGACCCGCTCCGTAG
5' Primer Tm (º C): 65.41 3' Primer Tm (º C): 61.56
PCR Condition: Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98°C 40 sec melt, 72°C 70 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (Thermo Scientific), GC buffer (Thermo Scientific), MgCl2 3 mM final concentration, 0.54M Betaine, 1.34mM DTT, 1.34% DMSO, 11ug/ml BSA, template 100ng/ul final concentration.
Nucleotide Sequence: ATGCTGCGCAAGCACACGTACTTCGACGAGCTCGCGCAGAACAAGCGCGCCTTCGCGGCGGCCGCCGCCGCCGCCTCGTC
GGCGGCGGCCACCACCTCGACGTCGCTGGGCAACGACAACCGTTCCTCCTCCCCCGCGTGCGCGCGGGAGCACCTCTTCG
ACAAGGCGGTCACCCCCAGCGACGTGGGCAAGCTGAACCGGCTGGTGATCCCGAAGCAGCACGCCGAGAGGCACTTCCCG
CTGCATCTCGCGGCCGCCGCCGGCGGCGGCGAGAGCACGGGCGTGCTCCTCAACCTGGAGGACGCCGCGGGGAAAGTGTG
GCGGTTCCGGTACTCGTACTGGAACAGCAGCCAGAGCTACGTGCTCACCAAGGGCTGGAGCCGCTTCGTCAAGGAGAAGG
GCCTCCAGGCCGGCGACGTCGTCGGCTTCTACCGCTCCGCGGCCGGCGCCGACAGCAAGCTCTTCATCGACTGCAAGCTG
CGACCCAACAGCGTGGACACCGCGTCGACGACGAGCCCCGTGGGGTCATCGCCTCCGCCGGCGCCGGTGGCGAAGGCCGT
GCGTCTCTTCGGCGTCGATCTGCTGACGGCGCCGGCGGACGCCATGGCCCGGTGCAAGAGAGCCAGGGACTTGGCCTCGC
CCCCGCAGGCGGCGATCAAGAAGCAGCTCGTGGAGCTGGCACTAGCTAGTGTAGATCAATGCTACGGAGCGGGTCGATCT
TTCTCT
TFome clones do not contain stop codon at 3'end.
Translation: MLRKHTYFDELAQNKRAFAAAAAAASSAAATTSTSLGNDNRSSSPACAREHLFDKAVTPSDVGKLNRLVIPKQHAERHFP
LHLAAAAGGGESTGVLLNLEDAAGKVWRFRYSYWNSSQSYVLTKGWSRFVKEKGLQAGDVVGFYRSAAGADSKLFIDCKL
RPNSVDTASTTSPVGSSPPPAPVAKAVRLFGVDLLTAPADAMARCKRARDLASPPQAAIKKQLVELALASVDQCYGAGRS
FS
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map