TFome Information for pUT6015

TFome Name:pUT6015
Genbank Insert ID:KJ727920
Protein Name:ZmbZIP58
Species: Maize
TF Family: bZIP
Template: BT041314.1
Corresponding TF Gene: GRMZM2G149040
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B462_F 3' Primer Name: B462_R
5' Primer Sequence: caccATGGCGATGCCACCGAA 3' Primer Sequence: GAAGGTGGTGTTGCTTTCACCCG
5' Primer Tm (º C): 68.6 3' Primer Tm (º C): 66.9
PCR Condition: PCR Conditions: Volumes per reaction (25l): 5 l GC buffer (Thermo Fisher) 1 l primer forward 20M 1 l primer reverse 20M 5 l glycerol 50% 1 l 1 ng/l DNA template 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (Thermo Fisher) 9.75 l ultrapure water PCR program 40 sec 98°C 30x : 10 sec 98°C 30 sec 65°C (annealing) 30 sec 72°C (extension) 10 min 72°C leave at 4°C
Nucleotide Sequence: ATGGCGATGCCACCGAAGCCCGGCGACCCGCCGCAGCGCTCGCCGGCACGTAGCCCAAACCTCAATCTCCCCTGCCCGCT
GCCGCCGGTCCCGGGCGGGGCGCCGCCACCGCCGCAGCAGCAGCCGGGCGCCGGGCTGCCCCCGCCGCGCGTGGGCCACC
ACCGCCGCGCCAGATCTGAGGTGGCCTTCCGCTTCCCGGACGACCTGCCCGGCGGCGGGGGCGGCGGCTTCGACGAGATC
GGCTCCGAGGACGACCTCTTCTCCACCTTCATGGACATGGACAAGATCGCCGGCGCCGACCGCGACCGCGCCGCCGAGAC
CTCCTCGCCGCCGCGCCCAGCCAAGCACCGCCACAGCGTCTCCTTTGACGGCTTCGGGATGGGGGCTGCCGCCGGCGGCC
CGGGCGGCCAGCAGGACGGCGGTGGAGGCATGTTCGGAGAGGTGATGGAGGCCAAGAAGGCCATGTCTTCCGAGCAGCTC
TCCGAGCTCGCCGCCATCGATCCCAAGCGCGCCAAAAGAATTATTGCAAACAGGCAATCTGCAGCACGGTCAAAGGAAAG
AAAAGCTCGATATATAACAGAATTGGAGCGGAAGGTTCAAACTCTTCAGACAGAGGCTACTACTCTTTCAGCACAGCTGA
CACTATTCCAGAGAGACACAACTGGACTTTCAGCAGAAAATGCTGAGCTCAAGATAAGGTTGCAGGCCATGGAGCAGCAG
GCCCAACTGCGTGACGCTCTAAATGATGCACTGAGGCAAGAAGTGGAGAGGCTTAAGATAGCAACGGGCGAGATGTCGAA
GTCCAATAACGAGCATTTCAATATGGGAATGCAGCACGTTTCCTACAGCCCTTCATTCTTCCAGCTCTCGGAGCAACACA
CAGTGCAGCAGCATGGAAACATTCAGCTGCCGCACCATTTCCAACAGCCTCCGCTGAATGTCCCGAGCCACCAGATGCTA
TCCCACCCCAACTCCCTCTCGAACATGGTGCAGCAAGACTCTCTTGGGCGTCTCCAGGGGCTGGACATTGGGAAAGGCTC
CATGGCTGTGAAGTCGGAGGCTGAGGTCGTGGTGAAGTCAGAGGGCAGCTCTATATCTGCGGGTGAAAGCAACACCACCT
TC
TFome clones do not contain stop codon at 3'end.
Translation: MAMPPKPGDPPQRSPARSPNLNLPCPLPPVPGGAPPPPQQQPGAGLPPPRVGHHRRARSEVAFRFPDDLPGGGGGGFDEI
GSEDDLFSTFMDMDKIAGADRDRAAETSSPPRPAKHRHSVSFDGFGMGAAAGGPGGQQDGGGGMFGEVMEAKKAMSSEQL
SELAAIDPKRAKRIIANRQSAARSKERKARYITELERKVQTLQTEATTLSAQLTLFQRDTTGLSAENAELKIRLQAMEQQ
AQLRDALNDALRQEVERLKIATGEMSKSNNEHFNMGMQHVSYSPSFFQLSEQHTVQQHGNIQLPHHFQQPPLNVPSHQML
SHPNSLSNMVQQDSLGRLQGLDIGKGSMAVKSEAEVVVKSEGSSISAGESNTTF
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis:
 
Plasmid Map