TFome Information for pUT6033

TFome Name:pUT6033
Genbank Insert ID:KJ727938
Protein Name:ZmbHLH88
Species: Maize
TF Family: bHLH
Template: BT062076
Corresponding TF Gene: GRMZM2G350165
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGCAGCCCACTACGCGGGAAATGCAGGCCATGGCCGCCGCCGGCCAGCAGATCTCCCTCGACGACCTCCGCGCGGCAGC
AGGCGGCGTGCACGACGACTTCCTGGACCAGATGCTCGGAGGCCTGCCGCCGTCGGCATGGCCGGAGCTGTCGTCCGCGG
CGGGAGGGAAGGCGCCGGATGGCGGCGCGCAGGCGGAGCAGATGCAGCACCAGCCGCAGCACTTGGGTGGGGGTGGGGGC
CTGTACGACGACTCCGCCTTGCTGGCGTCGCGGCTCCGGCAGCACCAAATCAGCGGCGGCGGCGGCGGGGAGGCCGTGAA
GCAGATGGTGCTGCAGCAGCTGGCCGATCTGAGGCAGGAACACCATGTGTTGCTGCAGGGCATGGGCCGCTCGACCTCGA
CGGGCGGCAGCAGAGACGGCGGCCTGCTCCTCCCGCTCTCTCTCGGCAGTGGCGGATCGGGCGGCGACGTGCAGGCGCTA
CTCAAAGCTGTAGCCGACTCCGCCGGAGGAGAAGCCGCAGGCGTCTTCGGCGGTTCCTTCGCCGGATCGCTCCACCACCA
CCAGCAGCAGCAGCAGCATTTTCAATCACATCCGCAGCAAACGGCGCCGTTGCCGGGGCAGGGCTTCGGTGGAGGCGCGG
GCGCGTCCGGCGGCGTGTCGCAGCCGCAGGCCGGCGCTGCGTGCGGAGGCGCCGCCGCGCCGCCCAGGCAGCGGGTACGG
GCTCGGCGCGGCCAGGCCACCGACCCCCACAGCATCGCGGAGCGGCTCCGGAGAGAGAGGATCGCGGAGAGGATGAAGGC
GCTTCAGGAGCTGGTGCCCAACGCCAACAAGACGGACAAGGCGTCGATGCTGGACGAGATCGTCGACTACGTCAAGTTCC
TTCAGCTCCAAGTCAAGGTGCTGAGCATGAGCCGGCTAGGTGGTGCCGCTGCCGTCGCGCCGCTGGTGGCCGACATGTCC
TCGGAGGGTCGAGGCGGCGTGGCGGTCGCGGCCGGGAGCGATGATGGGCTGGCGGTGACGGAGCAGCAGGTGGCGAAGCT
GATGGAGGAGGACATGGGCACCGCCATGCAGTACCTGCAAGGGAAGGGCCTGTGCCTCATGCCGGTCTCCCTCGCCTCCG
CCATATCCTCCGCGACGTGCCACATGCGACCTCCGGTGGGAGGACCGGGCCTCGGGGTCGCCGCCGCCGCCGCGCACCAC
ATGGCCGCTATGCGGTTGCCGCCCCACGCCATGAACGGCGGCGCCGGAGCCGGAGCCGACGCCGTGCCGGCCTCACCCAG
CATGTCCGTGCTCACGGCGCAGTCGGCCATGGCGCCCAACGGCGCCGGGGGTGGGACTGATGGCGAGGGCTCGCACTCGC
AGCAGCAGCAGCGGCATCATCCCAAGGACGCCGCGTCCGTGTCGAAGCCG
TFome clones do not contain stop codon at 3'end.
Translation: MQPTTREMQAMAAAGQQISLDDLRAAAGGVHDDFLDQMLGGLPPSAWPELSSAAGGKAPDGGAQAEQMQHQPQHLGGGGG
LYDDSALLASRLRQHQISGGGGGEAVKQMVLQQLADLRQEHHVLLQGMGRSTSTGGSRDGGLLLPLSLGSGGSGGDVQAL
LKAVADSAGGEAAGVFGGSFAGSLHHHQQQQQHFQSHPQQTAPLPGQGFGGGAGASGGVSQPQAGAACGGAAAPPRQRVR
ARRGQATDPHSIAERLRRERIAERMKALQELVPNANKTDKASMLDEIVDYVKFLQLQVKVLSMSRLGGAAAVAPLVADMS
SEGRGGVAVAAGSDDGLAVTEQQVAKLMEEDMGTAMQYLQGKGLCLMPVSLASAISSATCHMRPPVGGPGLGVAAAAAHH
MAAMRLPPHAMNGGAGAGADAVPASPSMSVLTAQSAMAPNGAGGGTDGEGSHSQQQQRHHPKDAASVSKP
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map