TFome Information for pUT6079

TFome Name:pUT6079
Genbank Insert ID:KJ727966
Protein Name:ZmbHLH48
Species: Maize
TF Family: bHLH
Template: RT-PCR
Corresponding TF Gene: GRMZM2G027068
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R22-F 3' Primer Name: R22-R
5' Primer Sequence: caccATGTTCCCGGTCGAAGTGTCGG 3' Primer Sequence: TGCTGCCGCTTGCATGTTCATG
5' Primer Tm (º C): 64.86 3' Primer Tm (º C): 61.34
PCR Condition:
Nucleotide Sequence: ATGTTCCCGGTCGAAGTGTCGGCGCTCGCGGCGGGCGGTGCGGGAGCGGGGCGTCAGGCTGCCGGCGACGCCGCCGGGAC
GATGCTGCCGCCCTTTTTCATGGGCTCCATCTGGCCCGCGACCGCTGGCGCCGCTGGCTCCGAGGAGGACGAGGCGGCGG
CCGCCGCCGCCGCCCACGACCGCGCGCTCGCCGCGTCGCGCAACCACCGCGAGGCCGAGAAGCGCCGTCGCGAGCGCATC
AAGTCGCACCTCGACCGCCTCCGCAACGTCCTCGCCTGCGACCCCAAGATAGACAAGGCTTCGCTGCTGGCGAAGGCGGT
GGAGAGGGTGCGGGACCTGAAGCAGCGGGCGGCGGGCGTCGGGGAGGCAGCGCCGGCGCACCTGTTCCCGACGGAGCACG
ACGAGATCGTTGTGCTCGCGTCGGGCAGCGGCGCCGTCTTCGAGGCCTCCGTCTGCTGCGACGACCGCTCCGACCTCCTC
CCGGACCTCATCGAGACGCTGCGCGCGCTCCGCCTCCGCACGCTCCGCTCCGAGATGGCCACCCTCGGCGGCCGCGTCCG
CAACGTGCTCGTCCTGGCGCGCGACGTCGTCGACGGCGGCGGCGGTGCCGTCGCGGGAGGTGATGACGGATACGGTGGAC
GCGCTGCTGACTCGGCGGGCGGCGCCACGGACGGCGGCGGCGGCGACTTCCTCAAGGAAGCCCTGCGGGCTCTGGTAGAG
AGGCCAGGCGCCGCCGGAGACCGGCCCAAGAGGCGACGGGTCTCGGACATGAACATGCAAGCGGCAGCA
TFome clones do not contain stop codon at 3'end.
Translation: MFPVEVSALAAGGAGAGRQAAGDAAGTMLPPFFMGSIWPATAGAAGSEEDEAAAAAAAHDRALAASRNHREAEKRRRERI
KSHLDRLRNVLACDPKIDKASLLAKAVERVRDLKQRAAGVGEAAPAHLFPTEHDEIVVLASGSGAVFEASVCCDDRSDLL
PDLIETLRALRLRTLRSEMATLGGRVRNVLVLARDVVDGGGGAVAGGDDGYGGRAADSAGGATDGGGGDFLKEALRALVE
RPGAAGDRPKRRRVSDMNMQAAA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map