TFome Information for pUT6084

TFome Name:pUT6084
Genbank Insert ID:KJ727970
Protein Name:ZmABI19
Species: Maize
TF Family: ABI3-VP1
Template: RT-PCR
Corresponding TF Gene: GRMZM2G035701
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R44-F 3' Primer Name: R44-R
5' Primer Sequence: caccATGGCCGGCATTACCAAGCGC 3' Primer Sequence: CATCTGAGGCCCGGATTTTGG
5' Primer Tm (º C): 66.73 3' Primer Tm (º C): 58.46
PCR Condition:
Nucleotide Sequence: ATGGCCGGCATTACCAAGCGCCGCACCTCCCCGGCCTCCACCTCCTCTTCGTCCGGCGACGTCTTGCCGCAGCGGGTCAC
CCGGAAGCGTCGGTCCGCCCGCCGCGGGCCCCGGAGCACCGCCCGTAGGCCGTCGGCGCCTCCACCTATGAATGAACTGG
ACTTGAATACAGCTGCTCTTGATCCGGATCATTATGCTACAGGATTGAGAGTTCTTCTTCAGAAGGAGCTCCGAAATAGC
GATGTAAGCCAGCTTGGGAGAATTGTTCTCCCAAAGAAGGAGGCGGAGTCTTACCTCCCTATTCTGATGGCAAAGGATGG
AAAGAGTTTATGCATGCATGACTTGCTAAATTCACAACTGTGGACCTTCAAGTATAGATATTGGTTCAACAACAAAAGCA
GGATGTATGTGCTTGAAAATACCGGAGATTATGTAAAAGCTCATGACCTTCAGCAAGGAGACTTCATCGTGATCTACAAG
GACGACGAGAACAACCGCTTTGTCATAGGAGCAAAGAAGGCAGGAGATGAGCAGACCGCCACTGTACCTCAAGTCCATGA
ACACATGCACATCTCTGCCGCACTGCCAGCTCCACAAGCGTTCCATGACTATGCAGGCCCCGTCGCAGCAGAAGCTGGTA
TGCTCGCGATCGTGCCACAGGGTGACGAGATATTCGACGGCATACTGAACTCCCTGCCGGAGATACCAGTGGCGAACGTG
AGGTACTCCGACTTCTTCGACCCGTTCGGTGACTCCATGGACATGGCGAATCCGCTGAGCTCCTCCAATAACCCCTCGGT
CAACCTGGCTACGCATTTCCATGACGAGAGGATCGGGAGCTGCTCGTTTCCCTACCCAAAATCCGGGCCTCAGATG
TFome clones do not contain stop codon at 3'end.
Translation: MAGITKRRTSPASTSSSSGDVLPQRVTRKRRSARRGPRSTARRPSAPPPMNELDLNTAALDPDHYATGLRVLLQKELRNS
DVSQLGRIVLPKKEAESYLPILMAKDGKSLCMHDLLNSQLWTFKYRYWFNNKSRMYVLENTGDYVKAHDLQQGDFIVIYK
DDENNRFVIGAKKAGDEQTATVPQVHEHMHISAALPAPQAFHDYAGPVAAEAGMLAIVPQGDEIFDGILNSLPEIPVANV
RYSDFFDPFGDSMDMANPLSSSNNPSVNLATHFHDERIGSCSFPYPKSGPQM
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map