TFome Information for pUT6109

TFome Name:pUT6109
Genbank Insert ID:KJ727989
Protein Name:ZmbHLH33
Species: Maize
TF Family: bHLH
Template: RT-PCR
Corresponding TF Gene: GRMZM2G015666
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R20-F 3' Primer Name: R20-R
5' Primer Sequence: caccATGGCACTGGAGGCCGTGGTGCTA 3' Primer Sequence: AGCTGCAGCCTTGTTTTGCTCTTCATTAATC
5' Primer Tm (º C): 69.08 3' Primer Tm (º C): 61.2
PCR Condition:
Nucleotide Sequence: ATGGCACTGGAGGCCGTGGTGCTATCCCAGTCCCAGTTCCAGCAAGGCAGTCGCTTTGGGTGCGGTGCCATGGCAGCAGG
AGGGGCTTGGAGTGATCTGTTGTTTAGTGGAACGGAGGGGTTGTTCGAGATGGGTGGCGCCGCCGGCCGCGGCTGGAACG
CGGCCGCGAGCTCGCCGCCGCAGCTCCTGCAGGAGCTGGGTGACAACGGTGCTGCCGGCTCACTACCTGTGGCGTCTGCG
GGGAGTGCGAGTGGCGGCGCCGGTCAGGATGCCCCGGCAATGGCTGCGGCAGCGGCGTCCGGAAGGAGGAAGCGGCGGAG
GATGAGGCCAGTGAAGAACGAGGAGGAAGTGGAGAGCCAGCGGATGATCCACATTGCGGTGGAGCGCAACCGCCGCAAGC
AGATGAACGAGCACCTCGCCGCGCTCCGCTCACTCATGCCGCCGGCCCACACGCAACGGGGCGATCAGGCATCCATCGTC
GGCGGCGCGATCAACTTCGTCAAGGAGCTGGAGCAGCTGCTGCAGTCGCTGGAGGCGCGGAGGCGTTCCCCGCAGTGCGC
TGCCTACGCCGTCGATCCCGACGACGCGGGGCCGTTCGCGGACTTCCTCACCTTCCCGCAGTACTCGATGTGCGCCGTCA
TTGCGGCCCCAGAGAACACTGGCCACCACCGTGAGGGCGGCGCCGTCGCCGAGCAGGAGGCGTCCGGGTCGAAGCCGTCG
GCCGTGGCCGACGTCGAGGCGACAATGGTGGAGAGCCACGCCAACCTGCGGGTGCTGTCCCGGCGCCGGCCGAGGCAGCT
GCTGCGCCTGGTGCTGGGGCTCCAGGGCCACCGCCTCACCGTGCTCCACCTCAACATGAGCAGCGGCGCCCACATGGTGC
TCTACTCGTTTAGCCTCAAGGTGGAGGATGATTGCCAGCTTACCTCCGTGGGTGAGATTGCGGCTGCGGCTCATCACATC
GTCGAGAAGATTAATGAAGAGCAAAACAAGGCTGCAGCT
TFome clones do not contain stop codon at 3'end.
Translation: MALEAVVLSQSQFQQGSRFGCGAMAAGGAWSDLLFSGTEGLFEMGGAAGRGWNAAASSPPQLLQELGDNGAAGSLPVASA
GSASGGAGQDAPAMAAAAASGRRKRRRMRPVKNEEEVESQRMIHIAVERNRRKQMNEHLAALRSLMPPAHTQRGDQASIV
GGAINFVKELEQLLQSLEARRRSPQCAAYAVDPDDAGPFADFLTFPQYSMCAVIAAPENTGHHREGGAVAEQEASGSKPS
AVADVEATMVESHANLRVLSRRRPRQLLRLVLGLQGHRLTVLHLNMSSGAHMVLYSFSLKVEDDCQLTSVGEIAAAAHHI
VEKINEEQNKAAA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map