TFome Information for pUT6139

TFome Name:pUT6139
Genbank Insert ID:KJ728006
Protein Name:ZmbHLH128
Species: Maize
TF Family: bHLH
Template: RT-PCR
Corresponding TF Gene: GRMZM2G314882
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R40-F 3' Primer Name: R40-R
5' Primer Sequence: caccATGATGAACTGCGCCGGAGG 3' Primer Sequence: AGCATTAGGCGGCCAGAGCTTTG
5' Primer Tm (º C): 64.22 3' Primer Tm (º C): 62.15
PCR Condition:
Nucleotide Sequence: ATGATGAACTGCGCCGGAGGCGGAGACCCCGTGGAGGACTTCCTGATCTCCGGCGCCGTCGACGACGAAGATCTAGCCAT
CTTCTGCGACGGAGGACTTGGGATTGAGGGTGTCAATGGAGATGCTTGTGGATTTGAGCAGTCTAATTTGGGCAAAAGGG
GTAGAGATGAACCATGTTCATCTGGTCTAAAATCCAAAGCTTGTCGTGAAAAAATGAGGAGGGACAAGCTGAATGACAGG
TTCCTGGAATTAAGTTCGGTTATGAATCCTGGAAAACAAGCAAAGTTGGATAAAGCCAATATCTTGAGCGACGCAGCCCG
TATGGTGGCACAACTTAGAGGTGAGGCAGAAAAGCTTAAAGAATCAAATGAGAAGCTGCGGGAGAATATCAAGGACCTGA
AGGAGGAGAAGAATGAGCTCCGCGAAGAGAAAGTGAGGCTGAAGGTAGAGAAAGATAGGCTGGAGCAGCAAGTCAAAGCC
ATGAGTGTAGCTCCTACAGGATATGTTCCCCATCTCCCTCATCCAGCTTCATACCACCCGGCTGCATTTGCTCCATTTGT
GCCGCCACAGCAAGCTGCAGGCAACAAAAGTGCTCCAATCCCTGCACCTTTCCCTGGGATGGCAATGTGGCAGTGGCTGC
CTCCGACCATCGTGGACACAACTCAAGACCCAAAGCTCTGGCCGCCTAATGCT
TFome clones do not contain stop codon at 3'end.
Translation: MMNCAGGGDPVEDFLISGAVDDEDLAIFCDGGLGIEGVNGDACGFEQSNLGKRGRDEPCSSGLKSKACREKMRRDKLNDR
FLELSSVMNPGKQAKLDKANILSDAARMVAQLRGEAEKLKESNEKLRENIKDLKEEKNELREEKVRLKVEKDRLEQQVKA
MSVAPTGYVPHLPHPASYHPAAFAPFVPPQQAAGNKSAPIPAPFPGMAMWQWLPPTIVDTTQDPKLWPPNA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map