TFome Information for pUT6140

TFome Name:pUT6140
Genbank Insert ID:KJ728007
Protein Name:ZmbHLH154
Species: Maize
TF Family: bHLH
Template: RT-PCR
Corresponding TF Gene: GRMZM2G385543
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: R42-F 3' Primer Name: R42-R
5' Primer Sequence: caccATGGCTTATAATGGACAGTATGGGTTTGG 3' Primer Sequence: CTGCTCGATCTTCATGGAGCCAGAGT
5' Primer Tm (º C): 61.78 3' Primer Tm (º C): 61.92
PCR Condition:
Nucleotide Sequence: ATGGCTTATAATGGACAGTATGGGTTTGGATCTTATGGCATGGGCTTAGAAGATAAACTGGGGTTGTATCCATCTTCATC
TGGTACACTGAGTCAGGACATCCAGATGAGTGAAGAGCATAGCGGAGTTGTGAAGAAGAGGAAAGGAGTGGAGGACTGTG
TCACATTGTTGCACGATGCAGGTGATCAACAAACAAAAGGTTCGCCTCAGCCAGAAAGGAATTCAGTGGAGGAGGGGAAC
CGAAAGATTTCGCCCAAAATGCAAAGCAAGGAAGATTCTTCTGATGGTGATGGTACTAAAGAGGACTATGTTCATATCCG
GGCAAAGCGAGGCCAAGCCACTAACAGCCACAGCCTTGCAGAAAGACTGAGGAGAAAGAAGATAAGTGAGAGGATGAAGC
TTCTTCAAGATCTTGTTCCTGGATGCAGTAAGATCACTGGCAAGGCAGTCATGCTCGATGAGATTATCAACTATGTGCAG
TCATTGCAACGACAAGTCGAGTTCTTGTCAATGAAACTCGCAACTGTTAACCCCGAGCTTGGCTTTGACATTGAGCAGAT
CATATCCAAACAAATGCTGCTTTCACAAGACAGACATCTTGCTTTCTATGGAGTCGAACCAGGATCAAGCGGCCTTACTG
GCCCATTTAACCAAGGAATCATGCAGCCAGAATTGATGTGCACCATTGCCAATCCCGTTGATGTTTTGCACGGAGCTATT
CACGACGCGTCAACAATGAACCATATACCTTCTATGTGGGAAGGACTTCAGAATATGCCTCAGATGAACTTCAACCCTGG
CGTGGCAGCCGATAGCGGCACCAACAACTCTGGCTCCATGAAGATCGAGCAG
TFome clones do not contain stop codon at 3'end.
Translation: MAYNGQYGFGSYGMGLEDKLGLYPSSSGTLSQDIQMSEEHSGVVKKRKGVEDCVTLLHDAGDQQTKGSPQPERNSVEEGN
RKISPKMQSKEDSSDGDGTKEDYVHIRAKRGQATNSHSLAERLRRKKISERMKLLQDLVPGCSKITGKAVMLDEIINYVQ
SLQRQVEFLSMKLATVNPELGFDIEQIISKQMLLSQDRHLAFYGVEPGSSGLTGPFNQGIMQPELMCTIANPVDVLHGAI
HDASTMNHIPSMWEGLQNMPQMNFNPGVAADSGTNNSGSMKIEQ
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map