TFome Information for pUT6163

TFome Name:pUT6163
Genbank Insert ID:KJ728026
Protein Name:ZmbHLH108
Species: Maize
TF Family: bHLH
Template: RT-PCR
Corresponding TF Gene: GRMZM2G119823
Transcript: T02
Vector: pENTR/SD/D-TOPO

5' Primer Name: R51F 3' Primer Name: R51R
5' Primer Sequence: caccATGGACAAGTACTGGGAGGAGACGC 3' Primer Sequence: GGTCTCGTAATAGCTCATCGAGCTGTG
5' Primer Tm (º C): 64.96 3' Primer Tm (º C): 60.17
PCR Condition:
Nucleotide Sequence: ATGGACAAGTACTGGGAGGAGACGCAGCGCTACCTGGAGTACGAGGAGCTCAGCATCTACTTGGAGGCGCAGGAGGACGC
CATGTCTTGCTACGACTCCAGCTCGCCGGACGGCTCCATCTCCCACTCGTCGTCGTCGGCGGCGGCGTGCGGCAACAAGA
ACATCCTGATGGAGCGCGACCGCCGCCGCAAGCTCAACGACAAGCTCTACGCGCTCCGCAGCGTCGTGCCGAACATCACC
AAGATGGACAAGGCGTCGATCATCAAGGACGCCATCGAGTACATCCAGCAGCTGCAGGTGGAGGAGCGGCGGGTGCTGCA
GGAGCTGCGCGTGCTCGACGACGACACCGCCGCCGCCGCCACCGCGCAGGTGGAGTGCTGCGACGTCGACGGGGGCCTGC
TGCGACGGGAAGCCGAGCGCGCGAAGAAGATGAAGCGCGCGCAGTCCGTCGCCAGCGGCGCGCAGTCTGCTCCGCCTCCG
CCCCCGCCGCCGCACGTCGAGGTGCTGGAGCTGCGCGTGTCGGAGGTGGGCGACCACGTGCTGGTGGTCAGCGTGACGTG
CCGCAAGCGACGCGACGCCATGGCCAGGGTGTGCCGCGCCATCGAGGACCTCCGGCTCCGTGTCATCACTGCCAACGTCA
CCTCCGTCGCCGGCTGCCACGTGCACACCGTCTTCGTCGAGGTAGATAAAATCGACCACATCCAAGTGAAGAACATGATT
GAGGCTGCTCTCACGTTGCTCGACGCCCCCGGAAGCCCCCACAGCTCGATGAGCTATTACGAGACC
TFome clones do not contain stop codon at 3'end.
Translation: MDKYWEETQRYLEYEELSIYLEAQEDAMSCYDSSSPDGSISHSSSSAAACGNKNILMERDRRRKLNDKLYALRSVVPNIT
KMDKASIIKDAIEYIQQLQVEERRVLQELRVLDDDTAAAATAQVECCDVDGGLLRREAERAKKMKRAQSVASGAQSAPPP
PPPPHVEVLELRVSEVGDHVLVVSVTCRKRRDAMARVCRAIEDLRLRVITANVTSVAGCHVHTVFVEVDKIDHIQVKNMI
EAALTLLDAPGSPHSSMSYYET
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: John Gray <jgray5@utnet.utoledo.edu>
 
Plasmid Map