TFome Information for pUT6242

TFome Name:pUT6242
Genbank Insert ID:KJ728076
Protein Name:ZmARF24
Species: Maize
TF Family: ARF
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G030710
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGGCCGGCATCGACCTGAACGATACCGTTGAGGAGGACGAGGAGGAGGCTGAGCCAGGCAACGCTTGCTCTCAGCAGAG
CAGGACCTCTAGCGCTGCTACCTTCCCTCCACCACCACCAAATCAGCCAAGGCCATCTGCTGCTGTGTGCCTCGAGCTTT
GGCATGCTTGCGCTGGCCCAGTTGCTCCACTTCCAAGAAAGGGCAGCGTGGTGGTGTACCTGCCACAGGGCCATATCGAG
CATCTTGGCGACGCTGCTGCTGCAGGCGGCGGCGCTCCACCACCAGTTGCACTTCCACCACATGTGTTCTGCAGGGTGGT
GGACGTGACCCTGCACGCTGATGCTTCTACCGACGAGGTGTACGCTCAGCTGGCTCTGGTTGCTGAGAACGAGGATGTGG
CTAGAAGGCTGAGGGGCAGGTCTGAGGATGGCTCTGCTGAGGATGGGGATGAGGGCGAGACTGTGAAGCAGAGGTTCAGC
AGGATGCCGCACATGTTCTGCAAGACCCTGACCGCTAGCGACACCTCTACTCACGGCGGCTTCTCTGTTCCAAGAAGGGC
TGCTGAGGACTGCTTCCCGCCACTGGATTACTCACAGCAGAGGCCGTCACAAGAGCTGGTGGCTAAGGATCTGCATGGCA
CCGAGTGGCGCTTCAGGCATATCTACAGAGGCCAGCCACGCAGGCATCTGCTGACTACTGGCTGGTCTGCCTTCGTGAAC
AAGAAGAAGCTGGTCAGCGGCGACGCCGTGCTTTTCCTTAGGGGCGATAATGGCGAGCTGAGGCTTGGCGTTAGAAGGGC
CGCTCAGCTGAAGAACGGCAGCGCTTTCCCAGCCCTGTACAACCAGTGCCTGAACCTGGGCTCTCTGCCAAACGTGGCCC
ATGCTGTGGCTACCAAGAGCGTGTTCCACATCTACTACAACCCGAGGCTGTCCCAGAGCGAGTTCATCATCCCGTTCAGC
AAGTTCATCAAGAGCTTCAGCCAGCCGTTCAGCGCCGGCTCTAGGTTCAAGGTGAAGTACGAGAGCGACGACGCTAGCGA
GAGAAGGTGCACCGGCATCATTGCTGGCATCGGCGACGCTGATCCAATGTGGCGCGGCTCTAAGTGGAAGTGCCTGATGG
TGAGGTGGGACGACGACGTGGACTTCAGGCAGCCAAACAGGATCAGCCCGTGGGAGATCGAGCTGACCTCTTCTGTGAGC
GGCAGCCACATGAGCGCCCCAAACGCTAAGAGGCTGAAGCCATGCCTGCCGCACGTGAACCCAGATTACCTGGTGCCAAA
TGGCAGCGGCAGGCCAGACTTCGCTGAGTCTGCTCAGTTCCACAAGGTGCTGCAGGGCCAAGAGCTGCTGGGCTACAGGA
CCCATGATAACGCCGCTGTGGCTACTAGCCAGCCATGCGAGGCTACCAACATGCAGTACATCGACGAGAGGTCCTGCAGC
AACGACGCCAGCAACATCATCCCAGGCGTTCCAAGGATCGGCGTGAGGACTCCACTTGGCAGCCCAAGGTTCAGCTACAG
GTGCTCTGGCTTCGGCGAGTCCCCAAGGTTCCAGAAGGTTCTCCAAGGGCAAGAGGTGTTCCATCCGTACAGGGGCACTC
TGGTGGACGCTAGCCTTAGCAACTCTGGCTTCCACCAGCAGGACGGCTCTCATGTGCCAACCCAGGCTTCTAAGTGGCAC
GCTCAGCTTCACGGCTGCGCTTTCAGAGGCCAACAGGCTCCAGCTGTGCCAAGCCAGTCAAGCTCTCCACCAAGCGTGCT
GATGTTCCAGAGGGGCGACCCAAAGATGAGCCCGTTCGAGTTCGGCCACTTCCACGTCAACAAGAAGGAGGACCGCAGGG
CCATGTTCGTTCATGCTGGCGGCATTGGCGGCACCGAGCAGACTACTATGCTGCAGGCTCACCATGTGTCTGGCGGCACT
GGCAACAGGGATGTGACCGTTGAGAAGTCCCACCCAGCTGTGGCTGCTGCTTCTGATAACCGCGAGGTGAGCAAGAACAG
CTGCAAGATCTTCGGCATCAGCCTGACCGAGAAGGTGCCGGCCATGAAGGAGAAGGGCTGCGGCGATATCAACACCAACT
ACCCGAGCCCGTTCCTGAGCCTGAAGCAGCAGGTTCCAAAGAGCCTGGGCAACAGCTGCGCTACCGTTCATGAGCAGAGG
CCAGTGGTGGCTAGGGTGATCGATGTGAGCACCGTGGACATGATGATC
TFome clones do not contain stop codon at 3'end.
Translation: MAGIDLNDTVEEDEEEAEPGNACSQQSRTSSAATFPPPPPNQPRPSAAVCLELWHACAGPVAPLPRKGSVVVYLPQGHIE
HLGDAAAAGGGAPPPVALPPHVFCRVVDVTLHADASTDEVYAQLALVAENEDVARRLRGRSEDGSAEDGDEGETVKQRFS
RMPHMFCKTLTASDTSTHGGFSVPRRAAEDCFPPLDYSQQRPSQELVAKDLHGTEWRFRHIYRGQPRRHLLTTGWSAFVN
KKKLVSGDAVLFLRGDNGELRLGVRRAAQLKNGSAFPALYNQCLNLGSLPNVAHAVATKSVFHIYYNPRLSQSEFIIPFS
KFIKSFSQPFSAGSRFKVKYESDDASERRCTGIIAGIGDADPMWRGSKWKCLMVRWDDDVDFRQPNRISPWEIELTSSVS
GSHMSAPNAKRLKPCLPHVNPDYLVPNGSGRPDFAESAQFHKVLQGQELLGYRTHDNAAVATSQPCEATNMQYIDERSCS
NDASNIIPGVPRIGVRTPLGSPRFSYRCSGFGESPRFQKVLQGQEVFHPYRGTLVDASLSNSGFHQQDGSHVPTQASKWH
AQLHGCAFRGQQAPAVPSQSSSPPSVLMFQRGDPKMSPFEFGHFHVNKKEDRRAMFVHAGGIGGTEQTTMLQAHHVSGGT
GNRDVTVEKSHPAVAAASDNREVSKNSCKIFGISLTEKVPAMKEKGCGDINTNYPSPFLSLKQQVPKSLGNSCATVHEQR
PVVARVIDVSTVDMMI
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map