TFome Information for pUT6313

TFome Name:pUT6313
Genbank Insert ID:KJ728147
Protein Name:ZmARF13
Species: Maize
TF Family: ARF
Template: Synthesized with codon optimization for maize
Corresponding TF Gene: GRMZM2G378580
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: 3' Primer Name:
5' Primer Sequence: 3' Primer Sequence:
5' Primer Tm (º C): 3' Primer Tm (º C):
PCR Condition:
Nucleotide Sequence: ATGACCAGCAGCGCTGCCGCCGCTGCAGCTGCTGCTCAAGTTGCTGGCGGCGGCGGTGGTGGCGAGGGTGATGCCGCTGC
TGCTGCTAGAGGCGGGGGCGGCACTGAGGATGGCATGTACACTGAGCTGTGGAACCTGTGCGCTGGCCCACTGGTTACTG
TTCCAAGGGTGGGCGACAAGGTGTACTACTTCCCACAGGGCCACATCGAGCAGGTTGAGGCTTCTACTAACCAGGTGGCC
GAGCAGCACATGCAGTTCTACGACCTGCCGTGGAAGATCCTGTGCGAGGTGATGAACGTCGAGCTGAAGGCCGAGCCAGA
CAACGACGAGGTGTACGCTCAGCTGACTCTGCTGCCAGAGAGCAAGCAGCCAGAGGAGAATGGCAGCAGCGAGGAGATGC
CAGCTTCTCCACCAGCTGCTCTTGCTAGGCCAAGGGTGCACAGCTTCTGCAAGACCCTGACCGCTAGCGACACCTCTACT
CACGGCGGCTTCTCTGTGCTTAGAAGGCACGCTGATGAGTGCCTGCCGCCACTGGATATGACTAGGCAGCCACCAACCCA
AGAGCTGGTGGCTAAGGATCTGCACGGCGTTGAGTGGCGCTTCAGGCATATTTTCAGGGGCCAGCCAAGGCGCCACCTTC
TTCAATCTGGCTGGTCCGTGTTCGTGAGCGCCAAGAGACTTGTTGCCGGCGACGCCTTCATCTTCCTGAGAGGCGATTCT
GGCGAGCTGAGGGTTGGCGTTAGAAGGGCTATGAGGCAGCAGGCCAACGTGCCAAGCAGCGTGATCAGCAGCCACTCTAT
GCATCTGGGCGTGCTGGCTACCGCTTGGCATGCTGTTAACACCGGCACCATGTTCACCGTGTACTACAAGCCGAGGACCA
GCCCAGCTGAGTTCGTGGTTCCATGCGACCGCTACATGGAGAGCCTGAAGAGGAACTACCCGATCGGCATGAGGTTCAAG
ATGAGGTTCGAGGGCGAGGAGGCTCCAGAGCAAAGGTTCACTGGCACCATCGTGGGCAACGTGGACCCAGATCAAGCTGG
CTGGGCTGAGTCTAAGTGGCGCTACCTGAAGGTGAGGTGGGACGAGGCTAGCAGCATTCCAAGACCTGAGAGGGTGAGCC
CGTGGCAGATTGAGCCAGCTGTTAGCCCGCCACCGATCAACCCACTGCCAGTGCATAGACCAAAGAGGCCGAGGTCTAAC
GCCGTGGCTTCTCTGCCAGAGTCTAGCGCCCCAACTAAGGAGGCCGCTCCAAAGGTTACCCTCGAGACTCAGCAGCACGC
TCTGCAGAGGCCACTTCAGACCCAGGATAACGTGGCCCCGAAGTCTGTGTTCGGCGACAACTCTGAGCTGGACAGCGCTC
ACAAGAGCAGCCTTAGGCCATCTGGCTTCGACCTGGACAAGAGCACCATCGGCATGCAGAGGAAGCTGGGCAGCGACATC
TGGATGAGGATGAACAGGCCAGACGGCTACAGCGAGATGCTGTCTGGCTACCAGCCACCAAATGAGGGCGCTAGGAACAG
CCAGGGCTTCTGCTCACTGCCAGACCAGATTGCTGCTGGCAGGCCGAACTTCTGGCACACCGTGAACGCTCACTACCAGG
ACCAGCAGGGCAACCACAATCTGTTCCCAGGCAGCTGGTCCATGATGCCAAGCTCTACTGGCTTCGGCATGAACAGGCAG
AGCTACCCGATGATCCAAGAGGTGGGCGGCATGAGCCAGAGCTGCACCAACACTAAGTTCGGCAACGGCGTGTACGCTGC
TTTGCCAGGCAGAGGCATTGACCGCTACCCATCTGGCTGGTTCGGCCATACAACTCCAGGCGGCAGAGTGGACGATGCTC
AGCCAAGGGTTATCAAGCCGCAGCCACTGGTGCTTGCTCATGGCGAGGCTCTGAAGATGAAGGGCAACAGCTGCAAGCTG
TTCGGCATCCACCTGGACAGCCCAGCTAAGAGCGAGCCACTGAAGTCTCCACCATCTGTGGCTACCCCAGCCGCCGAGAA
GTGGATGGCTGATGGCATCGACGCTGACAAGAGCCCAGAGCCACACAAGACCCCAAAGCAGCTTGGCGCTACTCAGGTGG
ACCCAGTTCCAGAGAGATGCCCACAAGCTTCTAGGGGCACCCAGTGCAAGTCTCAGGGCGGCTCTACTAGGTCCTGCAAG
AAGGTTCACAAGCAGGGCATGGCTCTGGGCAGATCTGTGGACCTGACCAAGTTCAACGGCTACACCGAGCTGGTGGCCGA
GCTGGACGAGATGTTCGATTTCAACGGCGAGCTTAAGGGCTGCAGCAAGGAGTGGATGGTGGTGTACACCGACTACGAGG
GCGACATGATGCTGGTGGGGGACGATCCATGGAACGAGTTCTGCAGCATGGTGCACAAGATCTTCGTGTACACCCGCGAG
GAGGTGCAGAGGATGAATCCAGGCGCTCTGAACAGCAGGCCAGAGGATTCTGGCCTGGCTAACTCTACCGAGAGGGGCTC
TGCTTCTACTGCTGCTGCTCGCGAGGCTCCAGGCTACCAATCTGCTAGCAGCCTGAACAGCGACAACTGC
TFome clones do not contain stop codon at 3'end.
Translation: MTSSAAAAAAAAQVAGGGGGGEGDAAAAARGGGGTEDGMYTELWNLCAGPLVTVPRVGDKVYYFPQGHIEQVEASTNQVA
EQHMQFYDLPWKILCEVMNVELKAEPDNDEVYAQLTLLPESKQPEENGSSEEMPASPPAALARPRVHSFCKTLTASDTST
HGGFSVLRRHADECLPPLDMTRQPPTQELVAKDLHGVEWRFRHIFRGQPRRHLLQSGWSVFVSAKRLVAGDAFIFLRGDS
GELRVGVRRAMRQQANVPSSVISSHSMHLGVLATAWHAVNTGTMFTVYYKPRTSPAEFVVPCDRYMESLKRNYPIGMRFK
MRFEGEEAPEQRFTGTIVGNVDPDQAGWAESKWRYLKVRWDEASSIPRPERVSPWQIEPAVSPPPINPLPVHRPKRPRSN
AVASLPESSAPTKEAAPKVTLETQQHALQRPLQTQDNVAPKSVFGDNSELDSAHKSSLRPSGFDLDKSTIGMQRKLGSDI
WMRMNRPDGYSEMLSGYQPPNEGARNSQGFCSLPDQIAAGRPNFWHTVNAHYQDQQGNHNLFPGSWSMMPSSTGFGMNRQ
SYPMIQEVGGMSQSCTNTKFGNGVYAALPGRGIDRYPSGWFGHTTPGGRVDDAQPRVIKPQPLVLAHGEALKMKGNSCKL
FGIHLDSPAKSEPLKSPPSVATPAAEKWMADGIDADKSPEPHKTPKQLGATQVDPVPERCPQASRGTQCKSQGGSTRSCK
KVHKQGMALGRSVDLTKFNGYTELVAELDEMFDFNGELKGCSKEWMVVYTDYEGDMMLVGDDPWNEFCSMVHKIFVYTRE
EVQRMNPGALNSRPEDSGLANSTERGSASTAAAREAPGYQSASSLNSDNC
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: This construct was synthesized by Life Technologies Corporation using codon optimization for maize.
 
Plasmid Map