TFome Information for pUT6484

TFome Name:pUT6484
Genbank Insert ID:KJ728208
Protein Name:ZmbZIP92
Species: Maize
TF Family: bZIP
Template: BT033247.1
Corresponding TF Gene: GRMZM2G077124
Transcript: T03
Vector: pENTR/SD/D-TOPO

5' Primer Name: B485_F 3' Primer Name: B485_R
5' Primer Sequence: caccATGGGAGTTCAGACAATGTCGTC 3' Primer Sequence: GAAAGTGGCGGAATTCGTTCG
5' Primer Tm (º C): 61 3' Primer Tm (º C): 60.3
PCR Condition: PCR Conditions: Volumes per reaction (25l): 5 l GC buffer (Thermo Fisher) 1 l primer forward 20M 1 l primer reverse 20M 5 l glycerol 50% 1 l 1 ng/l DNA template 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (Thermo Fisher) 9.75 l ultrapure water PCR program 40 sec 98°C 35x : 15 sec 98°C 30 sec 60°C 40 sec 72°C 7 min 72°C leave at 10°C
Nucleotide Sequence: ATGGGAGTTCAGACAATGTCGTCCCACTCCCACGGCGACGACGGCCGCCGCGGCCTCCCGCGGCAGGGCTCGGTCTACGG
CCTCACGCTGACCGAGGTGGAGACGCAGCTGGGCGAGCCGCTGCGGACCATGAACCTGGACGACCTCCTGCGCACCGTGC
TCCCGGCCTCCGCCGCCGCCGCCGCAGGCCCGCCCCCGCCCCCGGCCCCGGCGGCCAAGAAGACGGTGGACGAGGTGTGG
CGCGACATCCAGAGCGCCGGCGGCGGCGGCGGCGGCCGTCAGCCCTCCATGGGCGAGATGACGCTGGAGGACTTCCTCTC
CCGCGCCGGCGTCGCCGTCGATGCCGCCCCGCATTGGATGCACCAGTACCCGCAGCAGCAGCAGTACGCGCTGCCGCGGC
CCCTACCGCTTCCCGGCCCGGCTCTGGACGCCGCGTACCACGGCGACCGGCCAGGTGTTTTCCTCTCGCACTCGCAGGTG
GCCGGGCGGAAGCGCGCCGCCACGGGGGCCGTGGCCGGGGACGGCGTCGTGGAGAGGACGGTGGAGCGGCGGCAGAAGCG
CATGATCAAGAACCGCGAGTCCGCGGCGAGGTCCCGGGCCAGGAAGCAGGCGTACACCAACGAGCTGGAGAACAAGGTGG
CCCGGCTGGAGGAGGAGAACAAGCGTCTCATTGAGCTCAAGGTGCTGAAACCACTGGAGCCACTGGAGCCGCCACCGGCG
GAGCAGAACAAGAAGCAGCCGCCGCCGCAACCGAAGAGGAAGCAGCCGCAGCCACCGCCACCGCCTCCGCCGCCGATTCA
GCAGCTGCCGCCGAAGCACCGCCACGACCTCCGGCGAACGAATTCCGCCACTTTC
TFome clones do not contain stop codon at 3'end.
Translation: MGVQTMSSHSHGDDGRRGLPRQGSVYGLTLTEVETQLGEPLRTMNLDDLLRTVLPASAAAAAGPPPPPAPAAKKTVDEVW
RDIQSAGGGGGGRQPSMGEMTLEDFLSRAGVAVDAAPHWMHQYPQQQQYALPRPLPLPGPALDAAYHGDRPGVFLSHSQV
AGRKRAATGAVAGDGVVERTVERRQKRMIKNRESAARSRARKQAYTNELENKVARLEEENKRLIELKVLKPLEPLEPPPA
EQNKKQPPPQPKRKQPQPPPPPPPPIQQLPPKHRHDLRRTNSATF
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map