TFome Information for pUT6507

TFome Name:pUT6507
Genbank Insert ID:KJ728230
Protein Name:ZmALF4
Species: Maize
TF Family: Alfin-like
Template: BT061750.1
Corresponding TF Gene: GRMZM2G153087
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: C208_F 3' Primer Name: C208_R
5' Primer Sequence: caccATGGACAGGCACGGCTCG 3' Primer Sequence: CTCTCTGCTCCTCTTGTTGCTGCAA
5' Primer Tm (º C): 70.2 3' Primer Tm (º C): 67.6
PCR Condition: PCR Conditions: Volumes per reaction (25l): 5 l GC buffer (Thermo Fisher) 1 l primer forward 20M 1 l primer reverse 20M 1.25 ul DMSO 100% 1 l 1 ng/l DNA template 1 l dNTPs 10mM 0.25 l phusion taq (Thermo Fisher) 14.5 l ultrapure water PCR program 40 sec 98°C 30x : 10 sec 98°C 30 sec 60°C 60 sec 72°C 7 min 72°C leave at 10°C
Nucleotide Sequence: ATGGACGGAGGAGCCGGCTTTCCGGGCACGCAGCCGGTCTCCCGCTCGCCGGAGGACGTCTTCCGGGACTACCGCGCGCG
CCAGGCCGGCCTGATCAGGGCGCTCACCACCGATGTGGAGAAGTTCTACGTGATGTGCGACCCAGAGAAGGAGAACTTAT
GTTTATATGGACTTCCTAATGAGACATGGGAAATAAACTTGCCTGCTGAAGAGGTCCCTCCTGAACTGCCAGAGCCAGCT
CTTGGAATTAATTTTGCTCGTGATGGGATGGATGAAAAAGATTGGCTATCACTTGTTGCAGTGCATAGTGATTCTTGGCT
AATGTCTGTTGCATTTTATTTTGGAGCAAGGTTTGGATTCGATAAAGAATCCAGGAAACGTCTCTTCACCATGATCAATA
ACCTTCCCAGCATATATGAGGTTGTCACAGGAACAGCCAAGAAAGAGCCCAAAGAAAAAACTCCTAAAAGCAACATTAAG
ACTAACAAATCTGGCTCAAAGCCCTCGCGCCATGCGGAACCCAACTCAAGGGTCCCAAAGATGCCACCTCCAAAGGACGA
GGAGAGTGAAGAGGAGGAAGGGGAACCACAGGAAGACCAGGAGAGTGCGCTGTGTGGCGCATGTGGCCTAGGTTATGACG
ACTTCTGGATCTGCTGCGACCTATGCGAGACATGGTTCCACGGCAAGTGTGTTAAGATCACCCCAGCTAAAGCGGACCAC
ATCAAGCAGTATAAGTGCCCCTCCTGCACGGGAAGCAAGAGGGCCAAGGTT
TFome clones do not contain stop codon at 3'end.
Translation: MDGGAGFPGTQPVSRSPEDVFRDYRARQAGLIRALTTDVEKFYVMCDPEKENLCLYGLPNETWEINLPAEEVPPELPEPA
LGINFARDGMDEKDWLSLVAVHSDSWLMSVAFYFGARFGFDKESRKRLFTMINNLPSIYEVVTGTAKKEPKEKTPKSNIK
TNKSGSKPSRHAEPNSRVPKMPPPKDEESEEEEGEPQEDQESALCGACGLGYDDFWICCDLCETWFHGKCVKITPAKADH
IKQYKCPSCTGSKRAKV
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map