TFome Information for pUT6540

TFome Name:pUT6540
Genbank Insert ID:KJ728263
Protein Name:ZmbZIP59
Species: Maize
TF Family: bZIP
Template: gDNA
Corresponding TF Gene: GRMZM2G396632
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: SE0086_F 3' Primer Name: SE0086_R
5' Primer Sequence: caccATGCACCACCCTGCTGGT 3' Primer Sequence: AAATGGTCGGTCATCACCGTCG
5' Primer Tm (º C): 64.7 3' Primer Tm (º C): 59.6
PCR Condition: Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98°C 40 sec melt, 72°C 60 sec extend, 35 cycles; Step 3, 72°C 10 min extend 1 cycle; 1ul Phusion (Thermo Scientific), GC buffer (Thermo Scientific), MgCl2 3 mM final concentration,5% DMSO, template 50ng/ul final concentration.
Nucleotide Sequence: ATGCACCACCCTGCTGGTGAGGTAGTTGCCGGTGTTTTCCTCCCGTACCTGCCGCCCGCCGCTGCAGCAGCCACAGCGCC
TTTCGGTTTCGGAACCGGACCCCAATCCCATAACCACGCGCCAGCAGATGACGACGGTCACCTGCTGTTCCCGTGCAACG
ACGACAGTGACCTGCTGACGCTGCTGCTGCCGCCCTACTCCGCCGTGGGCTTCCACTACGCCGGCCAGCAGCCATTATTG
CTTCATCATCGCCATGACCATGACGACGACAAGGCCTCCCAAGCAGCCGAGAACCGACAGACCCACCGTCCGCGGCAGCT
GCTCGCCGAGGAGAGACGGCGGCGGAGGACGGCCTCCAACCGCGAGTCCGCGCGGCGGTCGCGCGTGCGCAAGCAGAAGC
AGCTCGGCCAGCTCTGGGACCAGGTCGTCCACCTCCGGGGCGACAGCCGCGACCTGCTCGACCGCCTCAACCGCGCCATC
AGGGACTGCGACCGCGTCATGCGCGACAACGCGCGGCTGCGTAACGAGCGGGCGGGGCTGCAGAGGAGGCTCCTGGACCT
CATCACCGACGGCGACGGTGATGACCGACCATTT
TFome clones do not contain stop codon at 3'end.
Translation: MHHPAGEVVAGVFLPYLPPAAAAATAPFGFGTGPQSHNHAPADDDGHLLFPCNDDSDLLTLLLPPYSAVGFHYAGQQPLL
LHHRHDHDDDKASQAAENRQTHRPRQLLAEERRRRRTASNRESARRSRVRKQKQLGQLWDQVVHLRGDSRDLLDRLNRAI
RDCDRVMRDNARLRNERAGLQRRLLDLITDGDGDDRPF
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map