TFome Information for pUT6560

TFome Name:pUT6560
Genbank Insert ID:KJ728281
Protein Name:ZmbHLH8
Species: Maize
TF Family: bHLH
Template: gDNA
Corresponding TF Gene: GRMZM2G038479
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: P05up 3' Primer Name: P05dn
5' Primer Sequence: caccATGCAGCATCAAGGTGCCATGAA 3' Primer Sequence: GGCACTCTCGTCCTGCTGCAGG
5' Primer Tm (º C): 63.54 3' Primer Tm (º C): 64.53
PCR Condition: Step 1, 98°C 3 min melt, 1 cycle; Step 2, 98°C 40 sec melt, 72°C 70 sec extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; 1ul Phusion (Thermo Scientific), GC buffer (Thermo Scientific), MgCl2 3 mM final concentration, 0.54M Betaine, 1.34mM DTT, 1.34% DMSO, 11ug/ml BSA, template 100ng/ul final concentration.
Nucleotide Sequence: ATGCAGCATCAAGGTGCCATGAATGAGCTCGTCTCCCAGGCATCCTTCTGCTCCCCTGCCTCCCCACCGTCCTTTTTCTC
GGCGGCGGTTGGCCACCACTCAATGCTCGACTTCGTGTCTTGCGGGGTACCCGAGCAGTGGTTCCTGGGCGAAGAAGCAC
TCGATAAGCCCATCCATGATGGCGCGGAGTGGGTGGCCGCCGGAGGCTCGCATGACTCGGCGGGCTCCGACCTCTCCAGC
AACCCGCCGGCTGCCGGGATCGTCCTGTCCGAGCGCGCGGCGAGGAGGCGGGGCAGGAAGCCCGGGCCCCGGTCCGACAA
CCCCGGAATCAGCCATGTGGAGGCGGAGCGGCAGCGGCGGGAGAAGCTCAACCGCCGGTTCTGCGACCTCCGGGCCGCGG
TGCCCACGGTGTCGCGGATGGACAAGGCGTCCCTGCTCGCCGACGCCGCCGCCTACATCGCCGAGCTACGCGGCCGCGTC
GAGCAGCTAGAGGCCGAGGCCAAGCAGCAGGTCGCGTCGAGGAAGCTGGGCGGCAACCCAGCCATGTGCCCGGCCTCGGG
GGGGCTCGAGGAGAAGCTGGAGGTGCGGATGGTCGGGCGTAATGCGGCGGCGTTGCGTCTGACCACGGCCTCGACACGCC
ACGCCCCCGCGCTCCTCATGGGCGCGCTGCGGTCGCTGGACCTGCCCGTGCACAACGCGTGCGTGAGCCGCGTCGGCGGC
TCGGCGACGGTGCAGGACGCCGTGGTGGACGTGCCCGCCGCGCTCCAGGACGAAGGCTGCCTCCGCGCCGCGCTGCTCCA
CGTCCTGCAGCAGGACGAGAGTGCC
TFome clones do not contain stop codon at 3'end.
Translation: MQHQGAMNELVSQASFCSPASPPSFFSAAVGHHSMLDFVSCGVPEQWFLGEEALDKPIHDGAEWVAAGGSHDSAGSDLSS
NPPAAGIVLSERAARRRGRKPGPRSDNPGISHVEAERQRREKLNRRFCDLRAAVPTVSRMDKASLLADAAAYIAELRGRV
EQLEAEAKQQVASRKLGGNPAMCPASGGLEEKLEVRMVGRNAAALRLTTASTRHAPALLMGALRSLDLPVHNACVSRVGG
SATVQDAVVDVPAALQDEGCLRAALLHVLQQDESA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map