TFome Information for pUT6566

TFome Name:pUT6566
Genbank Insert ID:KJ728287
Protein Name:ZmCA2P6
Species: Maize
TF Family: CCAAT-HAP2
Template: BT041906
Corresponding TF Gene: GRMZM5G829103
Transcript: T03
Vector: pENTR/SD/D-TOPO

5' Primer Name: B948_F 3' Primer Name: B948_R
5' Primer Sequence: caccATGATGAGCTTCAAGGGCCAC 3' Primer Sequence: CGCCTTGAGGAGCTCGC
5' Primer Tm (º C): 68.3 3' Primer Tm (º C): 59.2
PCR Condition: 5 l GC buffer (Thermo Fisher) 1 l primer forward 20M 1 l primer reverse 20M 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (Thermo Fisher) 9.75 l ultrapure water Initial PCR program: 40 sec 98°C 30x : 10 sec 98°C 30 sec 60°C (prior 58°C) 30 sec 72°C 30 min 72°C leave at 10°C
Nucleotide Sequence: ATGATGAGCTTCAAGGGCCACGAGGGGTTTGGCCAGGTGGCCGCCGCCGGTGCCGGGAGCCAGGCTGCCTCCCATGGTGG
AGCAGGCCCGCTGCCATGGTGGGCGGGGCCCCAGCTGCTGTTCGGCGAGCCGGCGCCCCCGTCACCGGAGGAGACGCGCC
GGGACGCCCAGTTCCAGGTCGTGCCGGGGGTTCAGGGCACGCCGGATCCAGCGCCGCCCAAGACAGGGACACCTGAGGTC
CTCAAATTCTCTGTCTTTCAAGGGAATTTGGAGTCGGGTGGTAAAGGAGAGAAAACCCCAAAGAACTCTACCACTATTGC
TCTTCAGTCACCGTTCCCAGAATACAATGGCCGTTTCGAGATTGGTCTTGGTCAATCTATGCTGGCCCCTTCCAATTATC
CTTGTGCTGACCAGTGCTATGGCATGCTTGCGGCTTATGGAATGAGATCGATGTCTGGTGGGAGAATGCTGTTGCCACTA
AATGCGACAGCTGATGCACCCATCTATGTGAATCCGAAGCAGTACGAAGGCATCCTCCGCCGTCGCCGTGCTCGCGCCAA
GGCGGAGAGCGAGAACAGGCTCGCCAAAGGCAGAAAGCCCTATCTCCACGAGTCGCGCCACCTCCACGCGATGCGTCGGG
TAAGAGGCACCGGCGGGCGCTTCGTCAACACGAAGAAAGAAGGGCGTGGCACGGGCGTTGCTTCGAACGGGGGCAGCAAG
ACGGCTGCAGCGGCACCGTCGCGCCTCGCCATGCCCCCTAGCTTCCAGAGTAGCGTCGCCAGCCTGTCTGGCTCCGACGT
GTCAAACATGTACAGCGGCGGCTTGGAGCAGCACCTTCGGGCGCCGCACTTCTTCACCCCGCTGCCACCCATCATGGAGG
ACGGCGACCACGGTGGTCCCCCCACCCGCATCTCCTCCTCCTTCAAGTGGGCAGCCAGCGACGGCTGCTGCGAGCTCCTC
AAGGCG
TFome clones do not contain stop codon at 3'end.
Translation: MMSFKGHEGFGQVAAAGAGSQAASHGGAGPLPWWAGPQLLFGEPAPPSPEETRRDAQFQVVPGVQGTPDPAPPKTGTPEV
LKFSVFQGNLESGGKGEKTPKNSTTIALQSPFPEYNGRFEIGLGQSMLAPSNYPCADQCYGMLAAYGMRSMSGGRMLLPL
NATADAPIYVNPKQYEGILRRRRARAKAESENRLAKGRKPYLHESRHLHAMRRVRGTGGRFVNTKKEGRGTGVASNGGSK
TAAAAPSRLAMPPSFQSSVASLSGSDVSNMYSGGLEQHLRAPHFFTPLPPIMEDGDHGGPPTRISSSFKWAASDGCCELL
KA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis:
 
Plasmid Map