TFome Information for pUT6567

TFome Name:pUT6567
Genbank Insert ID:KJ728288
Protein Name:ZmCADR3
Species: Maize
TF Family: CCAAT-DR1
Template: BT033302
Corresponding TF Gene: GRMZM2G147712
Transcript: T01
Vector: pENTR/SD/D-TOPO
Note: c.A117C p.(=) Not present in template FlcDNA.

5' Primer Name: B950_F 3' Primer Name: B950_R
5' Primer Sequence: caccATGGACCCGATGGACATCGT 3' Primer Sequence: ACTATCCATGTCAGGTCCGGTTT
5' Primer Tm (º C): 69 3' Primer Tm (º C): 57.9
PCR Condition: 5 l GC buffer (Thermo Fisher) 1 l primer forward 20M 1 l primer reverse 20M 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (Thermo Fisher) 9.75 l ultrapure water Initial PCR program: 40 sec 98°C 30x : 10 sec 98°C 30 sec 60°C (prior 58°C) 30 sec 72°C 30 min 72°C leave at 10°C
Nucleotide Sequence: ATGGACCCGATGGACATCGTGGGGAAGTCTAAGGAGGACGTTTCCCTCCCCAAATCAACAATGGTTAAGATAATTAAGGA
GATGCTTCCTCCTGATGTACGAGTGGCAAGAGATGCCCAGGATCTTCTTGTTGAATGCTGCGTAGAGTTCATCAATCTCC
TTTCGTCTGAATCCAATGAAGTGTGCAGCAGAGAAGAGAAGAAAACAATTGCTCCTGAGCATGTTATCAAGGCTCTAAGT
GATCTTGGCTTCAGAGAGTACATTGAGGAGGTTTATGCTGCGTATGAACAGCACAAACTCGAGACTCTGGATTCTCCAAA
AGCTGGCAAGTTCACTAGGATTGAGATGACAGAGGAAGAAGCTGTTGCTGAGCAACAAAGGATGTTTGCTGAGGCTCGAG
CAAGGATGAATAACGGAGCCCCCAAGCCAAAGGAGCCAGAACAAGAACCGCCACAGCTACCACAAGCACAACCCCAACTT
CAGCTGCATACTGAACCACAGCAACCCATGCAATCTCAAGTTCAGTTGCATTCGCAAACACAACACTACCTCCAACCTCA
ACTGCAGCTCCACCATCAGCCACAACAGCTCCCACAAGTGCAGCTGCACTCTCAGCCGCAGCTGCAACCCCAAGTGCATC
TGCACCCTCAGCCGCAGTTGCCCCCACAATTGCAGGTCCATCAGCAGCTGCAGCAACCCCCACAAGTGCAGGTCCACCAG
CAGCCCGAAGTGCAGCCCCAAGAAGCGCAGTTGCAATCATCAGCCCAGCAAACCTCCCAACCGCAACCTCAGGCACAACT
ACAATCACAGGGGCACTCACAAGCGCAGCTGCAAGCCGGACTGCTGGGTCAGTTGCAGACACAGGCGCAAACCGGACCTG
ACATGGATAGT
TFome clones do not contain stop codon at 3'end.
Translation: MDPMDIVGKSKEDVSLPKSTMVKIIKEMLPPDVRVARDAQDLLVECCVEFINLLSSESNEVCSREEKKTIAPEHVIKALS
DLGFREYIEEVYAAYEQHKLETLDSPKAGKFTRIEMTEEEAVAEQQRMFAEARARMNNGAPKPKEPEQEPPQLPQAQPQL
QLHTEPQQPMQSQVQLHSQTQHYLQPQLQLHHQPQQLPQVQLHSQPQLQPQVHLHPQPQLPPQLQVHQQLQQPPQVQVHQ
QPEVQPQEAQLQSSAQQTSQPQPQAQLQSQGHSQAQLQAGLLGQLQTQAQTGPDMDS
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis:
 
Plasmid Map