TFome Information for pUT6573

TFome Name:pUT6573
Genbank Insert ID:KJ728293
Protein Name:ZmCA2P16
Species: Maize
TF Family: CCAAT-HAP2
Template: BT063803
Corresponding TF Gene: GRMZM2G091964
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: C545_F 3' Primer Name: C545_R
5' Primer Sequence: caccATGAGCAGCATGGAGTCGC 3' Primer Sequence: AGCCTCGTTCTGTCGTAGCTGTAG
5' Primer Tm (º C): 68.4 3' Primer Tm (º C): 60
PCR Condition: 5 l GC buffer (Thermo Fisher) 1 l primer forward 20M 1 l primer reverse 20M 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (Thermo Fisher) 9.75 l ultrapure water Initial PCR program: 40 sec 98°C 30x : 10 sec 98°C 30 sec 60°C (prior 58°C) 30 sec 72°C 30 min 72°C leave at 10°C
Nucleotide Sequence: ATGAGCAGCATGGAGTCGCGGCCGGGCCGAACGAACCTGGTGGAGCCCATAGGGCACGGCGCCGCGCTGCCGTCCGGCGG
CCAGGCAGTGCAGCCGTGGTGGACGAGCTCCGGGGCTGTGCTCGGTGCAGTCTCGCCAGCCGTCGTGGCGGTGGCGCCCG
GGAGCGGGACGGGGATTAGCCTGTCGAGCAGCCCGGCAGGTGGTAGTGGTGGTGGCGGCGCGGCTAAAGGAGCCGCGAGT
GACGAGAGCAGCGAGGATTCACGGAGATCTGGGGAACCAAAAGATGGAAGCGCTAGTCAAGAAAAGAACCATGCCACATC
GCAGATACCCGCTCTGGCGCCAGAGTATTTGGCACCATACTCGCAGCTGGAACTGAACCAATCAATTGCTTCTGCAGCAT
ATCAGTACCCAGATCCTTACTATGCAGGCATGGTTGCTCCCTATGGAAGTCATGCTGTGGCTCATTTTCAGCTACCTGGA
CTAACTCAATCTCGAATGCCATTACCTCTTGAAGTATCCGAGGAGCCTGTTTATGTAAATGCCAAGCAGTACCATGGTAT
CTTAAGACGACGGCAGTCCCGTGCTAAGGCTGAACTTGAGAAAAAGGTGGTCAAAGCCAGAAAGCCATACCTTCACGAGT
CTCGTCATCAGCACGCGATGAGGAGGGCAAGAGGAAACGGGGGACGCTTCCTGAACACAAAGAAAAGTGACAGTGGTGCT
CCCAATGGAGGCGAAAACGCCGAGCATCTCCATGTCCCTCCCGACTTACTACAGCTACGACAGAACGAGGCT
TFome clones do not contain stop codon at 3'end.
Translation: MSSMESRPGRTNLVEPIGHGAALPSGGQAVQPWWTSSGAVLGAVSPAVVAVAPGSGTGISLSSSPAGGSGGGGAAKGAAS
DESSEDSRRSGEPKDGSASQEKNHATSQIPALAPEYLAPYSQLELNQSIASAAYQYPDPYYAGMVAPYGSHAVAHFQLPG
LTQSRMPLPLEVSEEPVYVNAKQYHGILRRRQSRAKAELEKKVVKARKPYLHESRHQHAMRRARGNGGRFLNTKKSDSGA
PNGGENAEHLHVPPDLLQLRQNEA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis:
 
Plasmid Map