TFome Information for pUT6581

TFome Name:pUT6581
Genbank Insert ID:KJ728301
Protein Name:ZmCADR16
Species: Maize
TF Family: CCAAT-DR1
Template: BT060559
Corresponding TF Gene: GRMZM2G012654
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: B944_F 3' Primer Name: B944_R
5' Primer Sequence: caccATGCCACGTAAGATCACCTTGG 3' Primer Sequence: AGCTACTCCATTCTCCTTGGCATC
5' Primer Tm (º C): 67.9 3' Primer Tm (º C): 58.6
PCR Condition: Volumes per reaction (25l): 5 l GC buffer (Thermo Fisher) 1 l primer forward 20M 1 l primer reverse 20M 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (Thermo Fisher) 9.75 l ultrapure water Initial PCR program: 40 sec 98°C 30x : 10 sec 98°C 30 sec 60°C (prior 58°C) 30 sec 72°C 30 min 72°C leave at 10°C
Nucleotide Sequence: ATGCCACGTAAGATCACCTTGGTCAAGGAGGGGGAGCCGCAGCCGGTGGACGCTGCTCAAGGGGAGCCCGCTCCGGCACC
GGCGGCACCGACGGCCGCGTTGACGGAGGCGGAGGTGGACGAGCTGCCCAGGGCCATCGTGCGCCGCCTTGTCAAGGACA
AGCTTTCCCACGTCGCCGGTGGGGAAGGAGCCGAGGTCATCGTCAACAAGGACGCCATGGCCGCGTTCGCCGAGAGCGCC
CGCATCTTCATCCACTATCTCTCCGCCACTGCCAATGATATGTGCAAGGAATCAAAGAGGCAGACCATCAACGCTGATGA
TGTCCTCAATGCTCTTGATGACATGGAGTTTTCGGAGTTTGTAGAGCCCCTTAGGACTTCACTGCAAGAGTTTAGAAACA
AAAATGCAGACAAAAGGTCAGAAGCAAAGAAACAACAGAAGGAAAAGAGAAGGAAATTAAATGAAGAGACACTTCCGCAG
AAAGAAAATGATCCAGCTGATGATGCCAAGGAGAATGGAGTAGCT
TFome clones do not contain stop codon at 3'end.
Translation: MPRKITLVKEGEPQPVDAAQGEPAPAPAAPTAALTEAEVDELPRAIVRRLVKDKLSHVAGGEGAEVIVNKDAMAAFAESA
RIFIHYLSATANDMCKESKRQTINADDVLNALDDMEFSEFVEPLRTSLQEFRNKNADKRSEAKKQQKEKRRKLNEETLPQ
KENDPADDAKENGVA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis:
 
Plasmid Map