TFome Information for pUT6635

TFome Name:pUT6635
Genbank Insert ID:KJ728353
Protein Name:ZmbZIP119
Species: Maize
TF Family: bZIP
Template: RT-PCR
Corresponding TF Gene: GRMZM2G120167
Transcript: T01
Vector: pENTR/SD/D-TOPO

5' Primer Name: CD0067_F 3' Primer Name: CD0067_R
5' Primer Sequence: caccATGATGATGGCCAACGCGAAGC 3' Primer Sequence: AGTACGAAGAGGCCCAGAGAGAGC
5' Primer Tm (º C): 70.5 3' Primer Tm (º C): 61.7
PCR Condition: PCR Conditions: per reaction (25l): 10 l GC buffer (Thermo Fisher) 1.5 uL DMSO 1 l dNTPs 10mM 32 l ultrapure water 0.5 l Phusion HOT START II (Thermo Fisher) 2 l primer forward 20M 2 l primer reverse 20M 1 lmaize shoot cDNA template 60 sec 98°C 5x : 10 sec 98°C 30 sec 72°C 1:20 sec 72°C 25x : 10 sec 98°C 30 sec 65°C 1:20 sec 72°C 10 min 72°C leave at 10°C
Nucleotide Sequence: ATGATGATGGCCAACGCGAAGCTCCACAAGCAGGCCCTGCTGCCGCCGCGGAGCCCCTTCCCGACGGCGGCGCCGTACGC
CGACCGCGGCCCAATCGCGCGGCCGCAGGTTGGCGCCACGCACCACCGCCACGGCCACCACCAGCGCACGTCGTCCGAGA
GCTTCATCGAGGAGCAGCCGCCGTCGTGGCTCGACGACCTGCTCAACGAGCCAGAGACGCCCGCGGCGCGGCAGAGCGGC
CGGGCTGGGCACCGCAGGTCGTCCAGTGACTCGTTTGCGTTGTTTGACGGTGGAGCTGCTGGTGCTGGTGCTTACGCCAA
TGGTTTCGAGGGGATGGGGAGAGGAGGTGGGCAACCTGCGCCGTGGGGCGGTGTGCAGGAGTACTATGCCAAGCTGGCCC
CGATTGGGAGGGCACATGGCCGGCCGTGGGAGCAAGGCATGCCGAATTCGGCGGGCTTCAGGCATGGTGGTGGCCTGCCA
ATGCCCACAAAAGATAAGGTTGGTGGGCATCATGGGCCACCGAAAGTGTCGAGGGAGCATGACCATGGCATGGACAAGAG
AACTGATGATGCTGGCCATGATCAAAAGGTTGGAGCCAAGGAGGGTGTGCCGCCGAAGCATGCACAGTCAGAGGCGGACA
ACAAGCGTGCTAAACAGCAATATGCTCAGAGGTCTCGTGTCCGGAAGCTGCAGTATATTGCAGAGCTTGAGAGTAGAGTC
CAGGCATTACAGTCAGAAGGGGTAGAAGTGTCTGCTGAAATGGAGTTCCTTAGCCAGCAGAATATCATGCTAGACCTGGA
AAACAAAGCCTTGAAGCAAAGAGTGGAGAGTCTAGCACAGGAACAACTTATTAAGCGTTTTCAACAGGAGATGTTTGAGC
GGGAAATTGGTCGTCTCAGAACAATATATCAGCAACAGCAGCAACAACAGGCTCCTGCTCTTGTTCGTAGTAACAGCAGG
GACCTTGATGTGCAGTTCGCCAACTTGTCTCTGAAACACAAAGACCCCAACACGGGCCGGGATGCTCTCTCTGGGCCTCT
TCGTACT
TFome clones do not contain stop codon at 3'end.
Translation: MMMANAKLHKQALLPPRSPFPTAAPYADRGPIARPQVGATHHRHGHHQRTSSESFIEEQPPSWLDDLLNEPETPAARQSG
RAGHRRSSSDSFALFDGGAAGAGAYANGFEGMGRGGGQPAPWGGVQEYYAKLAPIGRAHGRPWEQGMPNSAGFRHGGGLP
MPTKDKVGGHHGPPKVSREHDHGMDKRTDDAGHDQKVGAKEGVPPKHAQSEADNKRAKQQYAQRSRVRKLQYIAELESRV
QALQSEGVEVSAEMEFLSQQNIMLDLENKALKQRVESLAQEQLIKRFQQEMFEREIGRLRTIYQQQQQQQAPALVRSNSR
DLDVQFANLSLKHKDPNTGRDALSGPLRT
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis: Erich Grotewold <grotewold.1@osu.edu>
 
Plasmid Map