TFome Information for pUT6662

TFome Name:pUT6662
Genbank Insert ID:KJ728380
Protein Name:ZmALF6
Species: Maize
TF Family: Alfin-like
Template: BT038859.1
Corresponding TF Gene: GRMZM2G115424
Transcript: T03
Vector: pENTR/SD/D-TOPO

5' Primer Name: C206_F 3' Primer Name: C206_R
5' Primer Sequence: caccATGGACGGCGGGGGCTC 3' Primer Sequence: CGCCCTGACCCTCTTGTTGGTGC
5' Primer Tm (º C): 72.3 3' Primer Tm (º C): 70.1
PCR Condition: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l primer reverse 20mM 5 l glycerol 50% 1 l DNA template (c=1 ng/l) 1 l MgCl2 50mM 1 l dNTPs 10mM 0.25 l phusion taq (NEB M0530L, now replaced by F-530) 10.75 l ultrapure water PCR program: 40 sec 98°C 30x : 15 sec 98°C 30 sec 58°C 30 sec 72°C 7 min 72°C leave at 4°C
Nucleotide Sequence: ATGGACGGAGGAGCCGGCTTCCCTGGCACGCCGGTCCCGCGCTCGCCGGAGGACGTTTTCCGGGACTACCGCGCGCGCCA
GGCCGGCCTAATCAGGGCGCTCACCACCGATGTTGAGAAGTTCTACGTGATGTGCGACCCAGAGAAGGATAATTTATGTT
TATATGGACTTCCCAATGAGACATGGGAAGTAAACTTGCCTGCTGAGGAGGTTCCTCCTGAACTCCCAGAGCCAGCTCTC
GGAATTAATTTTGCTCGTGATGGGATGAATGAAAAAGATTGGCTATCACTTGTTGCAGTGCATAGTGATTCTTGGCTAAT
GTCTGTTGCATTTTATTTTGGAGCAAGGTTTGGATTCGACAAGGAATCCAGGAAACGTCTCTTCACCATGATCAATAATC
TTCCCAGCATATATGAGGTTGTCACAGGAACAGCCAAGAAAGAGTCCAAAGAAAAAACTCCTAAAAGCAGCAACAAGACT
AACAAATCTGGCTCAAAGCCTTCACGCCAGGTAGAACCCAACTCCAGGGTCCCAAAGATGCCACCTCCAAAGGACGAGGA
GAGTGAAGGGGAGGAAGGTGAACCACAGGAAGACCATGAGAGTGCGCTGTGCGGCGCATGTGGCCTAGGTTATGACGACT
TCTGGATCTGTTGCGACTTATGCGAGACATGGTTCCACGGCAAGTGTGTCAAGATCACCCCAAATAAAGCGGAGCACATC
AAGCAGTATAAGTGCCCCTCGTGCACAGGAAGCAAGAGGGCCAAGGCT
TFome clones do not contain stop codon at 3'end.
Translation: MDGGAGFPGTPVPRSPEDVFRDYRARQAGLIRALTTDVEKFYVMCDPEKDNLCLYGLPNETWEVNLPAEEVPPELPEPAL
GINFARDGMNEKDWLSLVAVHSDSWLMSVAFYFGARFGFDKESRKRLFTMINNLPSIYEVVTGTAKKESKEKTPKSSNKT
NKSGSKPSRQVEPNSRVPKMPPPKDEESEGEEGEPQEDHESALCGACGLGYDDFWICCDLCETWFHGKCVKITPNKAEHI
KQYKCPSCTGSKRAKA
Request Information: Deposited to ABRC.
PI responsible for TF ORF clone synthesis:
 
Plasmid Map