TFome Information for ZmMYB50
| TFome Name: | ZmMYB50 |
| Species: | Maize |
| TF Family: | MYB |
| Genbank ID of Template cDNA: | DR785526 |
| Corresponding TF Gene: | AC184769.3_FGT016 |
| Vector: | pENTR/D |
| Insert: | PCR amplified CDS from template |
| 5' Primer Name: | B33 UP | 3' Primer Name: | B33 DN |
| 5' Primer Sequence: | CACCATGGGAGTGGAGTCGGAG | 3' Primer Sequence: | CGGCCGTGTGACGACGCC |
| 5' Primer Tm (º C): | 61.4 | 3' Primer Tm (º C): | 64.8 |
| PCR Condition: | Step 1, 95°C 4 min melt, 1 cycle; Step 2, 95°C 30 sec melt, 60°C 1 min anneal, 72°C 2 min extend, 35 cycles; Step 3, 72°C 7 min extend 1 cycle; Step 4, 4°C hold. 1ul taq DNA polymerase (Eppendorf), Taq buffer (Eppendorf), MgCl2 3.0 mM final concentration, and enhancer, template 20pg/ul final concentration. | ||
| Nucleotide Sequence: | atgggagtggagtcggagtgcgacaggataaagggcccctggagccccgaggaggacgaggcgctgcggcgtctcgtgga gcgccacggcgcgcggaactggacggcgatcgggcgcggcatcccggggcggtcggggaagtcgtgccggctgcggtggt gcaaccagctgtcgccgcaggtggagcgccgccccttcacgcccgaggaggacgccgcgatcctggccgcgcacgcccgg ctgggcaaccggtgggccgccatcgcgcgccttctcccggggcgcaccgacaacgccgttaagaaccactggaactcgtc gctcaagcggaagctcgccaccgccacggcgacctccagcggcagcgatgctgagcgggcgtgcaagcgcgcaagccctg gcccggggagcccgacggcgtcggaccgcagcgacctcagccacggctgcggcgccgcgcaggtgttccgtcccgtcccg cgcgccggaggcttcgacgccatcagcgccgcagacgtgcgccctccgcccccgccagcagtcgcagacgaggacccgct cacctcgctctccctctcgctgccggggctggaccagtcgccgtcgggcttccaccacgacagcgcccggagccacttcc aggagctctccccctcgtcgtccccgtcgccgcagtctactccacccgccttccctccgcaggcggctccgtctcagtcg tcgtacgcgttcagcggcgagctggttgccgcgatgcaggaaatgatccgcgccgaggtgcgcaagtacatgtccggcgc ggggctccgcgccggatgcgggcccggggccgtgggcgaggtgtgcatgccgcagctggtggagggtgtgatgcgcgccg ccgcggagcgcgtcggcgtcgtcacacggccg | ||
| TFome clones do not contain stop codon at 3'end. | |||
| Translation: | mgvesecdrikgpwspeedealrrlverhgarnwtaigrgipgrsgkscrlrwcnqlspqverrpftpeedaailaahar lgnrwaaiarllpgrtdnavknhwnsslkrklatatatssgsdaerackraspgpgsptasdrsdlshgcgaaqvfrpvp raggfdaisaadvrpppppavadedpltslslslpgldqspsgfhhdsarshfqelspssspspqstppafppqaapsqs syafsgelvaamqemiraevrkymsgaglragcgpgavgevcmpqlvegvmraaaervgvvtrp | ||
| Request Information: | Dr. John Gray Biol. Sci Dept. University of Toledo, 2801 West Bancroft Street, Toledo, OH 43606 jgray5@utnet.utoledo.edu | ||