TFome Information for pUT3443

TFome Name pUT3443
Template DV532518
Genbank Insert ID KJ726898
Protein Name ZmMYB121
Species Maize
TF Family MYB
Gene ID GRMZM2G147346
transcript T01
Vector pENTR/SD/D-TOPO
Note
Request Information: Deposited to ABRC
5' Primer Name: B277_F 3' Primer Name: B277_R
5' Primer Tm (º C): 75.3 3' Primer Tm (º C):
5' Primer Sequence: caccATGGGGAGGGCGCCGTGCTG 3' Primer Sequence: CAAGACGCTCAAGTCCCTCAT
PCR Condition: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Nucleotide Sequence: ATGGGGAGGGCGCCGTGCTGCGAGAAGGAGGGGCTGCGGCGGGGCGCGTGGAGCGCCGAGGAGGACCAGCGCCTGGTGGCCTACGTCCGGCAGAACGGCCACCCCAACTGGCGCGCGCTGCCACGCCAGGCCGGCCTGCTCCGCTGCGGCAAGAGCTGCCGCCTCCGCTGGATCAACTACCTCCGCCCCGACATCAAGCGCGGCAACTTCACCCCCGACGAGGAGGCCCTCATCGTCCGCCTCCACCGCGAGCTCGGCAACAGGTGGTCAGCGATCGCGGCGCAGCTGCCGGGGCGCACCGACAACGAGATCAAGAACGTGTGGCACACCCACATCAAGAAGCGCCTGGAGGACGGCCACGGGGAGGAGAAGAAGGCGCGCAAAGGCAAGCCCGCCGCCAAGAAGGCCGCCGACGCCGTGGGCCGCAGCAGCGAGCACGGGCCGTTCCTGACCGCGTCGCCGGGGCTGTCGAGCAGCGGCGTCACGTTCTCCGCCGCGGCGGACTCCGCCGCGGCCGTGTCGTCGTCGGCCGACAACGCGGCCACCACCAGCCACCACCCGCAGCAGGTCGGCGCCAGCAAGGCGGAGTCGTCGACGGAGTTGGACAGCGGCCTCAGCTCCGCCGAGTTCCCGCCCCTCGACGACAGCTTCTGGTCGTCGGCGGACGTCGTGGACATGGGCCTCGGCGCCACGGACGAGGAGCTGGGCCTCGCGTGCCCGCCGCCGCTGTCGTCGTCGTCCACCAGGGACGAGGACATGGAGTTCTGGCTCAACATGCTGCTGGAGGCCGGCGACATGAGGGACTTGAGCGTCTTG
TFome clones do not contain stop codon at 3'end.
translation: MGRAPCCEKEGLRRGAWSAEEDQRLVAYVRQNGHPNWRALPRQAGLLRCGKSCRLRWINYLRPDIKRGNFTPDEEALIVRLHRELGNRWSAIAAQLPGRTDNEIKNVWHTHIKKRLEDGHGEEKKARKGKPAAKKAADAVGRSSEHGPFLTASPGLSSSGVTFSAAADSAAAVSSSADNAATTSHHPQQVGASKAESSTELDSGLSSAEFPPLDDSFWSSADVVDMGLGATDEELGLACPPPLSSSSTRDEDMEFWLNMLLEAGDMRDLSVL