| PCR Condition:
|
PCR Reaction Mix:5 l GC buffer (NEB) 0,5 l primer |
| Nucleotide Sequence:
|
ATGGAGAAGAGCCCGGGCGCCGCGCCGACATCATCGCCCGAGGAGGAGGTGGCGGCGGATGGCCGCGGGCACGTTGAGGACGCGACGGGGCAGAAGGAGCAGGCGACGCCGGTGGTGCTCAAGAAGGGGCCGTGGACGGCAGCGGAGGATGCCATGCTCATGGACCACGTCCGGCACCACGGCGAGGGGAACTGGAACGCAGTGCAGAGGGTGACCGGCCTGCTGCGCTGCGGCAAGAGCTGCCGCCTGCGCTGGACCAACCACCTCCGCCCCAACCTCAAGAAGGGCTCCTTCTCCCCCGACGAGGAGCTCCTCATCGCCCAGCTCCACGCGCAGCTCGGCAACAAGTGGGCGCGCATGGCCGCGCATCTGCCGGGGAGGACGGACAACGAGATCAAGAACTACTGGAACACGAGGACCAAGCGCCGCCAGCGCGCCGGCCTGCCTGTGCACCCGCCGCCAGAGGTGCAGCTCCAGCTCGCCCTCGCCAGGCGCTGCCGCTACGACGACTTCTCGCCGCTGCCGTCCCCGCAGGAGCTGTCCGGTGTCCAGGCGCTGGTGGGCGCCGCCGCCTCGGCCGGGTACGCCAGCTCCCGCCCGGCGCCCCTCGACCTGGCTCGGCAGCTCACCCAGACGACGAACCAGCAGACGGAGACTGTGCAGTTCCTCTCGCCGCCACCCTTCTCGGCGCCGTCGTCCCCGTGGGCGAGGCCGTTCGCCCGGAACGCGCAGTACTTCCAGCTCGCGCACTCGTCGTCGCCCGTGTCCCCGACGACGCCGGACCTGTCCCTCGGCTACGGCGCGCGCAGCGCCGGCGCCGACCAGAGCAGGCTCCCGCCGCTCTCGCCGTCCCCTTGCCCCAGAGTGGTGGAGCTCCCTTCAAACCAATACGCCCAACCGACGCCGCCGGCCTCGGCCGCCGCCACCGCACTTGGAGGTGGTGGCGCAGCGCTGCCGGATCATCAGAACGCGGCGAGCCTCGAGAAGATGCTGCGCGAGCTGCATGACGTCATCAAGGTCGACCCGCCGGCGCTGGTCCCGGCCAGCGGCGGCGCTGCCCTGGAGTGGCATGGTGGCGGAGGTGAG |
|
|
TFome clones do not contain stop codon at 3'end.
|
| translation:
|
MEKSPGAAPTSSPEEEVAADGRGHVEDATGQKEQATPVVLKKGPWTAAEDAMLMDHVRHHGEGNWNAVQRVTGLLRCGKSCRLRWTNHLRPNLKKGSFSPDEELLIAQLHAQLGNKWARMAAHLPGRTDNEIKNYWNTRTKRRQRAGLPVHPPPEVQLQLALARRCRYDDFSPLPSPQELSGVQALVGAAASAGYASSRPAPLDLARQLTQTTNQQTETVQFLSPPPFSAPSSPWARPFARNAQYFQLAHSSSPVSPTTPDLSLGYGARSAGADQSRLPPLSPSPCPRVVELPSNQYAQPTPPASAAATALGGGGAALPDHQNAASLEKMLRELHDVIKVDPPALVPASGGAALEWHGGGGE
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