TFome Information for pUT5755

TFome Name pUT5755
Genbank Insert ID KJ727803
Protein Name ZmALF10
Species Maize
TF Family Alfin-like
Gene ID GRMZM2G050495
transcript T01
Vector pENTR/SD/D-TOPO
Note
Request Information: Deposited to ABRC
5' Primer Name: B409_F 3' Primer Name: B409_R
5' Primer Tm (º C): 73.7 3' Primer Tm (º C):
5' Primer Sequence: caccATGGCGGACGCCGCCTCT 3' Primer Sequence: CTGCCTCGCCCTCTTGGAGCTGCAG
PCR Condition: 5 l GC buffer (NEB) 1 l primer forward 20mM 1 l pr
Nucleotide Sequence: ATGGACAGGCACGGCTCGGTTCGGCTGCCCACCCCCGAGGATGTCTTCACGGATTTCCGCGCCCGCCGTGACGGCATTCTCAAGGCACTCACCACCGATGTGGAGGAGTTCTACAAGTTGTGCGACCCAGAGTATGAGACCCTGTGCCTGTATGGACTACCTAACGAAACCTGGAAAGTGAACGTTCGTCCTGCGAAAGTTCCTCAACAACTACCAGAGCCAGCAGAGGGAATAAACATTCCTCGCGATTCAATGCCAAAGGAAGATTGGCTCTCGTTCGTAGCTGCACACAGTGATGCATGGTTGGTTGCTATGGCATTTCACTTTGGGGCTCTCTTCGGATTTGATAAAGACGCCAGGAGACGGCTCCACATGATGATCAATAATCATCCCACTGTATCTGAAGTTGTGATAGGGAGTGGGGAGAAGCAACCAAAAGCCTGCAACACAAATTACGAAACCAAGTCAAGTTCTATTAAGGAACCCAGTTCTAGCAGCAAGCTAGCAGAGGAGCCTTTGCCAAAGAAGGAGCGACAGATCATCAAAGAAGATGGAGGCGGCGAGGATGAAGCTTACCCATGCGGCACATGTGGAGGGATGTACTCGGAAAATGGAGTATTCTGGATTGGCTGTGACATTTGCGACAAGTGGTACCATGGTGACTGTGTTCGCATCACACCTGCGGAGGCAACGCACATCGATCAGTACAGTTGCCCCGCTTGCAGCAACAAGAGGAGCAGAGAG
TFome clones do not contain stop codon at 3'end.
translation: MDRHGSVRLPTPEDVFTDFRARRDGILKALTTDVEEFYKLCDPEYETLCLYGLPNETWKVNVRPAKVPQQLPEPAEGINIPRDSMPKEDWLSFVAAHSDAWLVAMAFHFGALFGFDKDARRRLHMMINNHPTVSEVVIGSGEKQPKACNTNYETKSSSIKEPSSSSKLAEEPLPKKERQIIKEDGGGEDEAYPCGTCGGMYSENGVFWIGCDICDKWYHGDCVRITPAEATHIDQYSCPACSNKRSRE