TFome Information for pUT6467

TFome Name pUT6467
Template RT-PCR
Genbank Insert ID KJ728193
Protein Name ZmMYB53
Species Maize
TF Family MYB
Gene ID GRMZM2G143328
transcript T01
Vector pENTR/SD/D-TOPO
Note
Request Information: Deposited to ABRC
5' Primer Name: CD0153_F 3' Primer Name: CD0153_R
5' Primer Tm (º C): 74.1 3' Primer Tm (º C):
5' Primer Sequence: caccATGACTACGAGCAGGGTGGCCAGGT 3' Primer Sequence: GTAATCGATCGGTTTTGACTCCCAG
PCR Condition: PCR Conditions: Volumes per reaction (30l): 6 l GC
Nucleotide Sequence: ATGACTACGAGCAGGGTGGCCAGGTCGTGCGGCCGCGGCAGCGACGATGAGCCGGCGGTGCGCAAGGGGCCGTGGACGCTGGAGGAGGACCTCATCCTCGTCAGCTACATCTCCCAGCACGGGGAGGGCTCCTGGGACAACCTCGCGCGCGCAGCTGGACTGAACCGCAACGGCAAGAGCTGCAGGCTGCGGTGGCTCAACTACCTGAGGCCGGGGGTGCGGCGCGGCAGCATCACGGCGGGGGAGGACACGGTCATCCGGGAGCTCCACGCGAGGTGGGGGAACAAGTGGTCCAAGATCTCCAAGCACCTCCCCGGCCGAACCGACAACGAGATCAAGAACTACTGGAGGACCAGGATCCAGCAGAAGAAGCAGCAGGGAGCCAAGACGACGCAGCAGCGGGAGCCGTCGACGACCGCCAGCTCCGGCGCCGGGGACGACTACTGGTGCACCAAGCCCGACCCCGACCAGCAAGCGCACTACTGCCTGCAGAAAGCCGCCATGGCAGCTGCGACTGCGACGACGAGCGCGGTCGTCGTCAGCAGCGAGGACGACGCGCCGTCGGCGGCGCTGACGAGCCAGGACAGCTCCGCCGCGGCCGGCGGAGACTGGTACATGCACCAGCAGCAGATGTGCTACCCGTACTGCTCCGAGCTGAGCTTTGTCGCCGCCGGCCACGATGAAACGGTCGGCCTGGACGCTGTGACGATGCAGTTCTTGTCGTCGCACTTCACTGCCAGCTTTTGGACGAACGGCGTGGATGATTTCTGGGAGTCAAAACCGATCGATTAC
TFome clones do not contain stop codon at 3'end.
translation: MTTSRVARSCGRGSDDEPAVRKGPWTLEEDLILVSYISQHGEGSWDNLARAAGLNRNGKSCRLRWLNYLRPGVRRGSITAGEDTVIRELHARWGNKWSKISKHLPGRTDNEIKNYWRTRIQQKKQQGAKTTQQREPSTTASSGAGDDYWCTKPDPDQQAHYCLQKAAMAAATATTSAVVVSSEDDAPSAALTSQDSSAAAGGDWYMHQQQMCYPYCSELSFVAAGHDETVGLDAVTMQFLSSHFTASFWTNGVDDFWESKPIDY