TFome Information for pUT6594

TFome Name pUT6594
Template RT-PCR
Genbank Insert ID KJ728314
Protein Name ZmMYB28
Species Maize
TF Family MYB
Gene ID GRMZM2G111117
transcript T01
Vector pENTR/SD/D-TOPO
Note
Request Information: Deposited to ABRC
5' Primer Name: CD0138_F 3' Primer Name: CD0138_R
5' Primer Tm (º C): 73.2 3' Primer Tm (º C):
5' Primer Sequence: caccATGGGGCGATcaccATGCTGTGAGA 3' Primer Sequence: GACCAAGGAAGGCGACTGTGCTGTC
PCR Condition: PCR Conditions: Volumes per reaction (30l): 6 l GC
Nucleotide Sequence: ATGGGGCGATCACCATGCTGTGAGAAGATTGGACTGAAGAAAGGTCCATGGACGCCGGAGGAGGATGAGAAGCTGCTTGCCTTCGTTGAGGAACATGGACACGGGAGCTGGCGGGCATTACCTGCGAAGGCAGGCTTGCAGAGGTGTGGGAAGAGCTGCAGGTTGAGGTGGACAAACTACCTGAGGCCGGACATCAAGAGGGGCAAGTTCAGCTTGCAAGAAGAACAGACCATCATCCAGCTTCACGCTCTTTTAGGCAACAGGTGGTCGGCCATTGCAACACATCTACCAAATCGCACGGACAACGAGATCAAGAACCACTGGAACACGCACCTCAAGAAAAGGCTGGCCAAGATTGGGATCGATCCTGTCACCCACAAATCTACCTGTGGCACTCTCACTGGCACCACGAACGACAGATCAGCCAAGGCCGCGGCAAGCCTCAGCCACATGGCACAATGGGAGAATGCCCGCCTCGAGGCTGAGGCACGGCTGGCTCGAGAATCGAAGACACGAACAGCAACACCGACGCCATCTGCACTCCATGCGCAGCCAATGGATCTACCTGCCTCTGCTGCTTCTCCATGGCTTGACGTGTTGCATGCTTGGCAGGGTGCAAAGATAGACCTGGAGTCACCTACCTCCACACTGACGTTTACAGGGAGCAATGGTGGCATGCTGCCAACCCCCAGGACCAACGGACCAGAGGTATCAGAAAGCAACTCCGCGATGTCGCATTATCAGATGAGCGATGAGTTGGAGGGTGAAGAAACCTATTGGCAGATCTTCAGCAAGCACCAAGTGCCGGAAGTGGACAGCAAGGAGAGTGAAGATGACTTCATTGGCTGTGAGGAGCCGTGGTTCTCAGGGATGGCTGGGGTTGGAGCTGGCATGCTGCTTGATGTATCCAATGAGCATGAGCTATCAGAATGCTGGGGTGAGTCCAGCAGTGGCCAAACTGTTGAGCACAGCAAGCAAGCATCCGATAAGGAGGACAAGGATTATTGGAATTGGGTCCTTGACAGAGTAAACTCAGAGCTGACAGCACAGTCGCCTTCCTTGGTC
TFome clones do not contain stop codon at 3'end.
translation: MGRSPCCEKIGLKKGPWTPEEDEKLLAFVEEHGHGSWRALPAKAGLQRCGKSCRLRWTNYLRPDIKRGKFSLQEEQTIIQLHALLGNRWSAIATHLPNRTDNEIKNHWNTHLKKRLAKIGIDPVTHKSTCGTLTGTTNDRSAKAAASLSHMAQWENARLEAEARLARESKTRTATPTPSALHAQPMDLPASAASPWLDVLHAWQGAKIDLESPTSTLTFTGSNGGMLPTPRTNGPEVSESNSAMSHYQMSDELEGEETYWQIFSKHQVPEVDSKESEDDFIGCEEPWFSGMAGVGAGMLLDVSNEHELSECWGESSSGQTVEHSKQASDKEDKDYWNWVLDRVNSELTAQSPSLV