| TFome pUT1106 | |
|---|---|
| Template | DR804210 |
| Genbank Insert ID | HQ858670 |
| Protein Name | ZmHB74 |
| Species | Zea mays |
| TF Family | Homeobox |
| Gene id | GRMZM2G087741 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT1106
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B29 UP |
| Temperature (ºC) | 57.3 |
| Sequence
|
caccATGGAGGATCTGTACAGC |
3' Primer
| Name | B29 DN |
| Temperature (ºC) | |
| Sequence
|
GGGTCCAATTGTGCCTGT |
PCR Condition
Step 1, 95C 4 min melt, 1 cycle; Step 2, 95C 30 se
Amino Acid Sequence
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MEDLYSIHPGISRVVGGAASEASVAGAGAGGPSPSDLTELMKAQIASHPRYPSLLSAYIECRKVGAHPHVTSLLEEVSRERRPDAGAGEIGVDPELDEFMDAYCRVLVRYKEELTRPFDEAASFLSSIQAQLSDLCSGGSSPAATATHSDDMMGSSEDEQCSGDTDVPDMGQEHSSHLGDHELKEMLLKKYSGCLSRLRSEFLKKRKKGKLPKDARTVLLEWWNTHYRWPYPTEEDKVRLAAMTGLDPKQINNWFINQRKRHWKPSEDMRFALMEGVAGGSSGTTLYFDTGTIGP
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGAGGATCTGTACAGCATCCACCCGGGGATCTCGCGGGTCGTCGGAGGCGCGGCGAGCGAGGCGTCCGTCGCCGGCGCCGGCGCCGGCGGGCCCTCGCCGTCGGATCTGACGGAGCTCATGAAGGCGCAGATCGCCAGCCACCCTCGCTACCCGTCCCTCCTCTCCGCCTACATCGAGTGCCGCAAGGTGGGAGCGCATCCGCATGTGACGTCGCTGCTGGAGGAGGTCAGCCGGGAGAGGCGCCCCGACGCCGGCGCCGGGGAGATCGGCGTCGATCCTGAGCTCGACGAATTCATGGACGCTTACTGCCGGGTGCTGGTGCGCTACAAGGAGGAGCTGACCCGGCCGTTCGACGAGGCCGCGTCGTTCCTGAGCAGCATTCAGGCGCAGCTCAGCGACCTCTGCAGCGGTGGAAGCTCGCCGGCGGCTACCGCCACGCACTCCGATGACATGATGGGCTCCTCTGAGGATGAGCAATGCTCAGGGGACACTGATGTGCCAGACATGGGGCAAGAACATAGCTCTCACTTAGGTGACCACGAGCTCAAGGAGATGCTTCTTAAGAAGTACAGTGGATGCCTCAGCCGTCTTCGTTCAGAGTTCCTGAAGAAGCGGAAGAAAGGGAAGCTACCGAAGGATGCTCGGACGGTGTTACTAGAGTGGTGGAACACGCACTACCGCTGGCCTTATCCTACGGAGGAAGACAAGGTGAGGCTCGCAGCAATGACCGGTCTCGACCCGAAGCAGATCAACAACTGGTTCATCAACCAGAGGAAGAGGCATTGGAAGCCATCTGAGGACATGCGGTTCGCGCTCATGGAGGGTGTTGCCGGTGGGTCTTCTGGGACGACACTCTACTTCGATACAGGCACAATTGGACCC
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