| TFome pUT1133 | |
|---|---|
| Template | DT652174 |
| Genbank Insert ID | HQ858677 |
| Protein Name | ZmNAC4 |
| Species | Zea mays |
| TF Family | NAC |
| Gene id | GRMZM2G079632 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | c.Start+9 |
| Request Information | Deposited to ABRC |
| Subgenome: | 1 |
TFome clone pUT1133
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B79 UP |
| Temperature (ºC) | 67.3 |
| Sequence
|
caccATGGCGGCCGTGCAGC |
3' Primer
| Name | B79 DN |
| Temperature (ºC) | |
| Sequence
|
GAAGAAGGGGAAGCCGCCGT |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MAAVQQQQQQQRRDAEAELNLPPGFRFHPTDEELVAHYLCARAAGRRPPVSIIAEVDLYRFDPWDLPERALFGRREWYFFTPRDRKYPNGSRPNRAAGSGYWKATGADKPVEHEGRTAGIKKALVFYHGKPPRGVKTEWIMHEYRLAEAGGARAKKSGAGTLRLDDWVLCRLYNKKNEWEKMQQQKEKEKKAMESEASLSHSHSDTRTPESEIDDDPFPELGSLPAFDDMGAAPAPAGVVLPKEEVEDFGDLGGDDWLAGINLDDLQMPGDAADFYGSMLVSPMAAKMEPDGGFPFF
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCGGCCGTGCAGCAGCAGCAGCAGCAGCAGCGCCGCGACGCGGAGGCGGAGCTCAACCTGCCCCCGGGCTTCCGGTTCCACCCGACGGACGAGGAGCTGGTGGCGCACTACCTCTGCGCGCGCGCCGCGGGGCGCCGCCCGCCGGTGTCCATCATCGCCGAGGTCGACCTGTACCGCTTCGACCCCTGGGACCTGCCGGAGCGCGCCCTCTTCGGGCGCCGCGAGTGGTACTTCTTCACGCCGCGGGACCGCAAGTACCCCAACGGCTCCCGCCCCAACCGCGCCGCCGGGTCGGGCTACTGGAAGGCCACGGGCGCGGACAAGCCCGTGGAGCACGAGGGCCGGACGGCGGGGATCAAGAAGGCGCTCGTGTTCTACCACGGCAAGCCGCCGCGCGGGGTCAAGACGGAGTGGATCATGCACGAGTACCGCCTCGCCGAAGCCGGCGGCGCCCGCGCCAAGAAGTCCGGCGCCGGCACGCTCAGGCTGGATGACTGGGTGCTGTGCCGCCTGTACAACAAGAAGAACGAGTGGGAGAAGATGCAGCAGCAGAAGGAGAAGGAGAAGAAGGCGATGGAGTCGGAGGCGTCGCTCTCGCACTCCCACTCCGACACGCGGACGCCGGAATCGGAGATCGACGACGACCCGTTCCCGGAGCTGGGCTCTCTACCGGCGTTCGACGACATGGGCGCAGCCCCAGCCCCTGCCGGCGTCGTCCTGCCCAAGGAGGAGGTGGAGGACTTCGGCGACCTCGGCGGCGACGACTGGCTCGCGGGCATCAACCTCGACGACCTGCAGATGCCGGGGGACGCCGCCGATTTCTACGGCTCCATGCTCGTCTCGCCGATGGCCGCCAAGATGGAGCCGGACGGCGGCTTCCCCTTCTTC
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