TFome clone pUT1135

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B85 UP
Temperature (ºC)	60.0
Sequence Copy to clipboard	caccATGGCGACCGGC

3' Primer

Name	B85 DN
Temperature (ºC)
Sequence Copy to clipboard	CAGGGACCAGTTCATCGC

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MATGGGSSRSDDVRGLKFGKKIYFEQDGGSGSGAGAVGGRKGKGVATGGARPASAASAAQPPRCQVDGCGVDLSAVKQYYCRHKVCNMHSKEPRVFVAGIEQRFCQQCSRFHQLHEFDQGKRSCRRRLIGHNERRRKPPPGPLTSRYGRLAASLQEPGRFRSFLLDFSYPRVPSSVRDAWPGIQHGGDRMLGTVQWHGHQEPPHPHRSAAAGYGNHAAYNCHGGLVAGGAPMLSSAAFELPPGGCVAGVAADSSCALSLLSTQPWDTTSHDHRSPAMPAAGAFDGTPVAPSVMASSYAASSAWTGSRDPAADGARNAQRLDDALHLVHPGSAAVHFSGELELALQGSGGPPHLPRVDHGGSGGGTFNHSTTSAMNWSL

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCGACCGGCGGCGGCAGCAGCAGGAGCGACGACGTGCGCGGGCTCAAGTTTGGCAAGAAGATCTACTTCGAGCAGGACGGCGGGAGCGGGAGCGGGGCGGGGGCGGTGGGCGGCAGGAAGGGGAAGGGCGTGGCCACCGGTGGCGCGAGGCCGGCGTCCGCCGCCTCCGCAGCCCAGCCGCCGAGGTGCCAGGTGGACGGGTGCGGCGTGGATCTGAGCGCCGTCAAGCAGTACTACTGCCGGCACAAGGTGTGCAACATGCACTCCAAGGAGCCGCGCGTCTTCGTCGCCGGCATCGAGCAGCGCTTCTGCCAACAGTGCAGCAGGTTCCACCAGCTACATGAATTTGACCAAGGGAAACGTAGCTGCCGCCGCCGCCTCATCGGTCACAACGAGCGCCGGAGGAAGCCACCACCTGGACCTCTCACTTCACGATATGGCCGGCTCGCTGCATCACTTCAAGAGCCTGGCAGGTTCAGAAGCTTCCTGCTCGACTTCTCGTACCCAAGGGTTCCAAGCAGCGTGAGGGATGCGTGGCCAGGAATCCAGCACGGTGGCGACAGGATGCTGGGCACCGTCCAGTGGCATGGGCACCAAGAACCTCCTCACCCACACCGCAGTGCAGCTGCTGGCTATGGCAACCATGCTGCATACAACTGCCATGGCGGCTTGGTAGCAGGCGGGGCCCCAATGCTCTCCTCTGCCGCCTTTGAGCTCCCGCCTGGCGGATGTGTCGCGGGAGTTGCCGCCGACTCCAGCTGTGCTCTCTCTCTTCTGTCAACTCAGCCATGGGACACGACCTCCCACGACCACCGGTCCCCAGCAATGCCCGCGGCCGGCGCCTTCGACGGCACCCCGGTGGCGCCGTCCGTCATGGCGAGCAGCTACGCGGCGTCGAGCGCCTGGACGGGCTCGCGGGACCCCGCTGCCGACGGCGCCAGGAACGCGCAGCGTCTCGACGATGCTCTGCACCTGGTCCACCCAGGCTCCGCGGCGGTCCACTTTTCCGGCGAGCTCGAGCTCGCCCTGCAGGGAAGCGGCGGGCCGCCACACCTGCCGCGCGTCGACCATGGCGGCTCCGGCGGCGGCACCTTCAACCATTCCACCACCAGCGCGATGAACTGGTCCCTG