| TFome pUT1140 | |
|---|---|
| Template | EE011225 |
| Genbank Insert ID | HQ858684 |
| Protein Name | ZmbZIP95 |
| Species | Zea mays |
| TF Family | bZIP |
| Gene id | GRMZM5G858197 |
| transcript | T02 |
| Vector | pENTR/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT1140
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C25 UP |
| Temperature (ºC) | 62.5 |
| Sequence
|
caccATGGCATCGTCGAGGGTG |
3' Primer
| Name | C25 DN |
| Temperature (ºC) | |
| Sequence
|
CCACTGCATTGAGTTCGTtc |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MASSRVMPSSSPSHTASDLARLAQAASRPGGGGSGLGSMNVEELLRGIYSDMPTPAPPASEPERPRSPAPAPEVATRRTAEEVWKEITGGGGSGEPVPPVAAQAAVPAGGGGAGGPEMTLEDFLAREEGAVKEDGVRVSGPSAPAMGFLGGAEGVGVPGGGGGRGRKRQLMDPVDRAAMQRQKRMIKNRESAARSRERKQAYIAELESLVTHLEEENAELLREQEERHQKRLKELLERVTPVILRKKPSGDLRRTNSMQW
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGCATCGTCGAGGGTGATGCCGTCCTCCTCGCCGTCGCACACGGCCTCTGATCTCGCGCGCCTAGCGCAGGCCGCCAGCAGGCCCGGCGGCGGGGGCAGCGGGCTGGGCTCCATGAACGTGGAGGAGCTCCTCCGCGGCATCTACAGCGACATGCCCACCCCGGCGCCGCCCGCGTCGGAGCCCGAACGCCCGAGGTCGCCGGCCCCGGCGCCGGAGGTCGCCACGCGCAGGACGGCGGAGGAGGTGTGGAAGGAGATCACCGGCGGAGGTGGCAGTGGGGAACCCGTGCCCCCCGTGGCGGCTCAGGCTGCCGTCCCCGCTGGTGGTGGTGGCGCCGGCGGCCCGGAGATGACGCTGGAGGACTTCCTGGCCAGGGAGGAGGGCGCGGTGAAGGAGGATGGGGTTAGGGTTTCGGGCCCCTCTGCGCCGGCCATGGGGTTCCTGGGCGGAGCGGAGGGCGTGGGTGTGCCTGGCGGCGGAGGCGGGAGGGGAAGGAAGCGGCAGCTCATGGATCCAGTGGATCGCGCCGCGATGCAGCGACAGAAACGAATGATCAAAAACCGCGAGTCTGCTGCCCGGTCACGGGAGAGGAAGCAGGCTTATATCGCCGAGCTAGAATCGCTGGTAACGCACCTCGAGGAGGAGAATGCCGAGTTGTTGAGAGAACAGGAAGAGCGGCACCAGAAGCGGCTTAAAGAGCTCTTGGAACGGGTGACGCCAGTCATTCTGAGGAAAAAACCATCAGGAGATCTTAGAAGAACGAACTCAATGCAGTGG
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