| TFome pUT1144 | |
|---|---|
| Template | EE177571 |
| Genbank Insert ID | HQ858688 |
| Protein Name | ZmNAC133 |
| Species | Zea mays |
| TF Family | NAC |
| Gene id | GRMZM2G094067 |
| transcript | T01 |
| Vector | pENTR/D-TOPO |
| Note | |
| Request Information | Deposited to ABRC |
TFome clone pUT1144
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | C36 UP |
| Temperature (ºC) | 64.0 |
| Sequence
|
CAC CAT GGG AGG AGG AGC CAC C |
3' Primer
| Name | C36 DN |
| Temperature (ºC) | |
| Sequence
|
ATTATTTGGCAAGCTCACttcg |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MGGGATNLPPGFHFFPSDEDLVVHFLRRKAANLPCRPDIVPTILLHRYDPWELNGTALQAGNQWYFFSSHAHAHAHSAAGQTRTSPSGYWNPAGADETVVLASSGRVVGLKKTLVFCTGEPFRGFRTNWVMHEYHLQDGPAGCNVGGSSGATTPSSSSSSGSSRRSQRKRVHSSTESINSWVICRVFESSCGSQVSFHDEGTELSCLDEVFLSLDDYDEVSLPNN
TFome clones do not contain stop codon at 3'end.
CDS
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ATGGGAGGAGGAGCCACCAACCTACCTCCGGGCTTCCATTTCTTCCCTTCAGACGAAGACCTCGTCGTCCATTTCCTCCGCCGCAAAGCCGCCAACCTCCCGTGCCGCCCCGACATCGTCCCGACGATCCTCCTTCACCGCTACGACCCGTGGGAGCTGAACGGCACGGCGCTGCAGGCTGGGAACCAGTGGTACTTCTTCAGTAGCCACGCCCACGCCCACGCCCACTCCGCGGCGGGGCAGACCAGAACCTCTCCGTCCGGGTACTGGAACCCCGCCGGCGCCGATGAAACAGTAGTACTAGCGAGCAGCGGCCGTGTCGTTGGCCTCAAGAAGACGCTCGTCTTCTGCACCGGAGAGCCTTTCAGAGGGTTCAGAACGAACTGGGTCATGCATGAGTACCACCTTCAGGACGGGCCGGCCGGGTGCAATGTCGGCGGGAGCAGCGGCGCCACCACCCCAAGTAGCTCCAGCAGCTCCGGCAGCAGCAGGAGATCGCAGAGGAAGAGAGTTCACTCGAGCACGGAGTCAATAAACAGCTGGGTGATATGTAGAGTGTTCGAATCAAGCTGCGGTTCGCAAGTGAGCTTCCATGACGAGGGCACAGAGCTTTCGTGCTTGGATGAGGTGTTCTTGTCTCTAGACGACTACGACGAAGTGAGCTTGCCAAATAAT
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