| TFome pUT1155 | |
|---|---|
| Template | DR785591 |
| Genbank Insert ID | HQ858699 |
| Protein Name | ZmbHLH5 |
| Species | Zea mays |
| TF Family | bHLH |
| Gene id | GRMZM2G116785 |
| transcript | T02 |
| Vector | pENTR/D-TOPO |
| Note | c.Start+105. As present in template EST. |
| Request Information | Deposited to ABRC |
| Subgenome: | 2 |
TFome clone pUT1155
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B34 UP |
| Temperature (ºC) | 59.6 |
| Sequence
|
caccATGCCACCGTCAACCTAT |
3' Primer
| Name | B34 DN |
| Temperature (ºC) | |
| Sequence
|
CCGTTCAGGAGGGACTGC |
PCR Condition
Step 1, 95C 4 min melt, 1 cycle; Step 2, 95C 30 se
Amino Acid Sequence
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MPPSTYFMTARSDHNLNFSVHEQDSTMLPNEQLTYAGAGQIDLLPAETLSRDKLPENLLELQGLQNISNLQSNLVAPGVFQHNLTPGVFHPQLNTPGLSELPHALSSSIDSNGSEVSAFLADLNAVSSAPTLCSTFQNASSFMEPVNLEAFSIQGAQSDCVLNRTSHPNGNISVCDSAALASLHDSKEFISGSLPSFASVQETNLAASGFKTQKQEQNAVCNIPTFTARNQMSVAAMQGALVPQKIPSRINDNKSEGPVSHPSDVQTQPNSAVNGVGVKPRVRARRGQATDPHSIAERLRREKISDRMKNLQDLVPNSNKADKASMLDEIIDYVKFLQLQVKVLSMSRVGAPGAVLPLLTESKTEGYHGQPLPQGLLDALDSEDSFVFEEEVVKLMETSITSAMQYLQSKGLCLMPVALASAISTQKGVSAAAVPPER
TFome clones do not contain stop codon at 3'end.
CDS
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ATGCCACCGTCAACCTATTTCATGACAGCTAGATCAGATCACAATTTAAATTTCAGTGTGCATGAACAGGATTCCACTATGCTTCCAAACGAACAGTTGACCTATGCTGGTGCTGGCCAGATTGATCTATTACCTGCTGAGACTCTATCTAGGGATAAGCTTCCTGAAAATCTTCTGGAGCTTCAAGGACTTCAGAATATCAGCAACCTACAGAGTAATTTAGTGGCCCCAGGGGTGTTTCAGCACAATTTAACACCTGGGGTCTTTCATCCACAGTTGAACACTCCTGGACTTTCAGAACTGCCTCATGCCTTATCTAGTTCAATTGACAGTAATGGAAGTGAAGTTTCTGCTTTTCTCGCTGATCTAAACGCTGTTTCTTCAGCGCCAACTTTATGTTCCACATTCCAAAACGCTTCTTCGTTCATGGAACCAGTGAATCTAGAAGCTTTCAGTATTCAAGGCGCACAAAGTGATTGTGTTTTGAACAGAACAAGTCATCCGAATGGGAATATCTCAGTATGTGACAGTGCTGCCTTGGCATCACTACATGATAGCAAGGAGTTTATCAGTGGTAGTCTCCCTTCTTTTGCTAGTGTCCAGGAAACAAACCTAGCTGCTAGTGGCTTCAAGACTCAGAAGCAGGAGCAAAATGCAGTGTGCAATATCCCTACTTTCACTGCACGGAATCAAATGTCAGTTGCTGCAATGCAAGGAGCACTTGTCCCTCAAAAGATTCCTTCACGGATCAATGATAATAAAAGTGAGGGTCCTGTTAGCCATCCTTCTGATGTACAAACTCAACCAAATTCAGCTGTTAATGGTGTTGGTGTGAAACCACGAGTAAGGGCTCGTCGTGGACAAGCAACTGATCCTCATAGTATTGCTGAACGGCTTCGCAGAGAGAAAATTTCAGATAGGATGAAAAATCTGCAGGATCTTGTCCCAAATTCCAATAAGGCAGACAAGGCATCCATGCTGGATGAAATTATTGATTATGTGAAGTTTCTTCAGCTTCAGGTCAAGGTCTTGAGCATGAGTAGGGTAGGAGCTCCAGGAGCAGTTCTTCCCCTTCTCACAGAATCTAAGACTGAGGGTTACCACGGCCAACCTCTGCCACAAGGATTACTGGACGCACTAGACTCGGAAGACAGCTTCGTCTTCGAGGAAGAGGTCGTGAAGCTAATGGAGACCAGCATCACCAGCGCGATGCAGTACCTTCAGAGCAAGGGGCTCTGCCTGATGCCAGTCGCCCTTGCTTCCGCAATATCCACCCAGAAAGGTGTGTCCGCTGCTGCAGTCCCTCCTGAACGG
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