| TFome pUT1161 | |
|---|---|
| Template | DV531704 |
| Genbank Insert ID | HQ858705 |
| Protein Name | ZmGLK67 |
| Species | Zea mays |
| TF Family | G2-like |
| Gene id | GRMZM2G400489 |
| transcript | T02 |
| Vector | pENTR/D-TOPO |
| Note | c.start+279 Support in RNA-seq |
| Request Information | Deposited to ABRC |
TFome clone pUT1161
This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.
5' Primer
| Name | B74 UP |
| Temperature (ºC) | 65.0 |
| Sequence
|
CAC CAT GAC GCG GCG GTG C |
3' Primer
| Name | B74 DN |
| Temperature (ºC) | |
| Sequence
|
AACTGCATGAATTGGACTgctg |
PCR Condition
Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s
Amino Acid Sequence
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MTRRCSHCSNNGHNSRTCPVRSAGGGSGGGGVRLFGVRLTTAPAPAVMKKSASMSCIVSSLGGGFGGSSPPVEGVGAGRGGEDSGTGYVSDDPAHASCSTNGRAERKKGTPWTEEEHRMFLMGLQKLGKGDWRGISRNFVVSRTPTQVASHAQKYFVRQTNSSRRKRRSSLFDMVPEMPMDESPAAVEQFTLQNTQDEDTSSNHVPTLHLGQRKETEFAKQLQTFQPGHHEKSEYAEPSLLLPDLEMNPSVPFKTITDPTGPAFYPSLVPVPLTLWPPSVSHVEEAARTHEILKPTPLNGKGVVKADDVVGMSKLSIDEASSGSMEPTALSLELIGSTDTRQSAFHVSPPMNRPELSKRNSSPIHAV
TFome clones do not contain stop codon at 3'end.
CDS
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ATGACGCGGCGGTGCTCGCACTGCAGCAACAACGGCCACAACTCGCGTACCTGCCCCGTCCGCTCCGCCGGCGGCGGCAGCGGCGGCGGCGGGGTGAGGCTGTTCGGCGTGCGGCTCACGACGGCGCCGGCGCCGGCGGTGATGAAGAAGAGCGCCAGCATGAGCTGCATTGTGTCCTCGCTCGGCGGCGGTTTCGGCGGCTCGTCGCCGCCGGTGGAAGGAGTGGGTGCTGGCAGAGGAGGGGAAGACAGTGGGACCGGCTACGTGTCCGATGATCCCGCGCACGCATCCTGCTCGACGAATGGCCGCGCCGAGCGCAAGAAAGGTACACCTTGGACTGAAGAAGAGCATAGAATGTTTCTGATGGGTCTGCAGAAGCTTGGTAAGGGAGACTGGCGCGGGATATCCAGAAACTTTGTTGTTTCCAGGACCCCGACTCAAGTTGCAAGCCATGCTCAAAAGTACTTTGTTAGACAGACAAACTCATCGAGACGGAAGAGGCGGTCAAGCTTATTTGACATGGTCCCAGAAATGCCAATGGATGAGTCCCCAGCTGCTGTGGAACAATTTACTCTCCAAAATACTCAAGATGAAGATACAAGTTCAAATCACGTGCCAACCTTACATCTTGGACAACGGAAGGAAACAGAGTTTGCTAAGCAGCTACAAACTTTTCAGCCAGGGCACCATGAGAAATCTGAATACGCAGAACCTTCATTGCTGCTACCAGATTTGGAGATGAACCCCAGTGTACCATTCAAGACCATAACTGATCCAACAGGACCAGCATTCTACCCATCATTGGTCCCTGTTCCACTAACTCTTTGGCCTCCAAGTGTTTCCCACGTGGAGGAAGCAGCCAGAACCCATGAAATCCTAAAACCAACTCCTTTGAATGGTAAGGGGGTGGTTAAGGCAGATGATGTTGTTGGTATGTCTAAGCTCAGCATCGACGAAGCTAGCTCTGGCTCCATGGAACCCACTGCTCTTTCCCTTGAGCTTATTGGATCCACAGATACAAGGCAATCAGCTTTTCATGTGAGTCCACCGATGAATAGACCTGAACTAAGCAAGAGAAACAGCAGTCCAATTCATGCAGTT
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