TFome clone pUT1163

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B24 UP
Temperature (ºC)	64.0
Sequence Copy to clipboard	caccATGGACTCGACCCCTCCC

3' Primer

Name	B24 DN
Temperature (ºC)
Sequence Copy to clipboard	GGGTTCGCTCTTGGCAGCAT

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MDSTPPTDPAASCLASASAAVASGDVAAMLPDSPPRRGAGHRRAQSEILLGGAALPDDLTFDADLGVVGEACVAGDEDEEDDDDDEEGVGGAGGSRMFEMFLENGGKLPGPPEPSAHPHSTPPRPRHHHSMSMDGSTSLFGSAAAGTPGRSGADAKKAISDAKLAELALVDPKRAKRILANRQSAARSKERKMRYIAELERKVQTLQLEATTLSAQLAMLQRDTTGMTSENSDLKIRVQTMEQQVQLQDALNDRLRDEIQQLKVATGQVNASIGKTGNSGMSSFGGNPQSYQRSHIQSLLAAQHLQRLRIHSQHQQQQTHFQQQQQPQPQLLLQEAHPFPVDLKMRGFSLSSHAQNAGASDSHAAKSEP

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGACTCGACCCCTCCCACGGACCCGGCGGCCTCCTGCTTGGCCTCCGCCTCTGCGGCGGTGGCATCGGGGGACGTCGCCGCGATGCTGCCGGACTCGCCGCCGCGGCGTGGGGCGGGCCACCGCCGCGCACAGTCGGAGATCCTCCTCGGCGGCGCCGCGCTCCCCGACGACCTCACCTTCGACGCGGACCTCGGCGTTGTCGGGGAGGCCTGCGTGGCCGGCGACGAGGATGAGGAGGATGACGACGACGACGAGGAAGGGGTCGGCGGCGCTGGCGGCAGCCGGATGTTCGAGATGTTCCTCGAGAACGGTGGCAAGCTCCCAGGACCGCCGGAGCCGTCGGCGCATCCGCATTCGACGCCGCCGCGGCCACGGCACCATCACAGCATGTCTATGGATGGGTCCACGTCTTTGTTTGGTTCCGCCGCCGCCGGGACGCCTGGGAGGTCGGGCGCTGATGCCAAGAAGGCTATTTCCGACGCTAAGCTCGCCGAGCTCGCGCTTGTTGACCCCAAACGTGCCAAGAGGATTTTGGCAAACCGCCAGTCAGCTGCTAGGTCGAAAGAAAGAAAGATGCGCTACATTGCGGAGCTTGAAAGGAAAGTACAGACTCTCCAATTAGAAGCAACTACACTATCAGCTCAGCTGGCCATGTTGCAGCGAGATACGACTGGTATGACAAGTGAGAATAGTGATCTGAAGATACGTGTGCAGACAATGGAGCAGCAGGTTCAGCTGCAGGACGCTCTGAACGACAGGCTAAGAGACGAGATTCAGCAGCTGAAGGTTGCAACAGGACAGGTAAACGCCAGCATTGGAAAAACAGGAAACTCTGGTATGTCATCATTTGGAGGTAATCCACAAAGTTACCAGCGTAGCCATATACAGTCTCTTCTGGCTGCTCAGCACCTGCAGCGGCTCCGGATCCACTCCCAACATCAGCAACAGCAGACGCATTTTCAGCAGCAGCAGCAGCCGCAGCCGCAGCTGCTGCTGCAGGAGGCGCATCCATTTCCGGTGGACCTAAAGATGAGAGGATTTTCTCTGTCATCACATGCTCAGAACGCAGGTGCATCAGACAGCCATGCTGCCAAGAGCGAACCC