TFome clone pUT1171

This clone is an ORF (open reading frame).
The TFome is the collection of ORFs.

5' Primer

Name	B64 UP
Temperature (ºC)	58.2
Sequence Copy to clipboard	caccATGGCAGATGCTAGTTCG

3' Primer

Name	B64 DN
Temperature (ºC)
Sequence Copy to clipboard	CTCTCGTGGCCTAGCAAG

PCR Condition

Step 1, 98C 30 sec melt, 1 cycle; Step 2, 98C 10 s

Amino Acid Sequence

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MADASSRTDTSTVVDMDDKNQRLENGQSGAMVPASNSSDRSDRSDKPMDQKVLRRLAQNREAARKSRLRKKAYVQQLESSKLKLASLEQELQKARQQGIFISSSGDQTHTMSGNGAMTFDLEYSRWQEEQNKQINELRTAVNAHASDSDLRLIVDGIMAHYDEIFRLKGIAAKADVFHILSGMWKTSAERCFLWLGGFRSSELLKLLVNQLEPLTEQQLMGLSNLQQSSQQAEDALSQGMEALQQSLAETLAGSLGPSGSSGNVANYMGQMAMAMGKLGTLENFLRQADNLRQQTLHQMQRILTIRQAARALLAIHDYFSRLRALSSLWLARPRE

TFome clones do not contain stop codon at 3'end.

CDS

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ATGGCAGATGCTAGTTCGAGGACTGACACCTCGACTGTTGTAGACATGGATGATAAGAATCAAAGGTTAGAAAACGGACAGAGTGGAGCCATGGTCCCGGCTTCTAATTCATCTGACCGGTCTGACAGATCTGACAAACCTATGGACCAGAAGGTTTTACGACGGCTTGCCCAAAATCGTGAGGCAGCAAGAAAAAGCCGGCTGAGGAAAAAGGCATATGTGCAACAGCTAGAGAGCAGTAAGCTAAAACTTGCAAGCCTGGAGCAAGAACTCCAGAAAGCTCGACAGCAGGGAATCTTCATTTCTAGTTCTGGAGATCAAACTCATACTATGAGTGGAAATGGAGCTATGACCTTCGATTTAGAATATTCTCGATGGCAAGAGGAACAAAATAAGCAGATAAATGAGCTGAGGACTGCAGTAAATGCTCATGCAAGTGATAGTGACCTCCGTCTTATAGTCGATGGGATAATGGCACATTATGACGAGATATTCAGGCTGAAGGGCATTGCTGCAAAGGCTGATGTGTTCCATATACTTTCAGGCATGTGGAAAACATCTGCTGAAAGGTGCTTCTTGTGGCTTGGGGGTTTCCGTTCGTCTGAGCTTCTAAAGCTCCTTGTGAATCAGCTTGAGCCTCTAACTGAGCAGCAATTGATGGGGCTGTCCAATCTGCAGCAATCTTCTCAGCAGGCTGAGGATGCATTGTCACAAGGAATGGAAGCATTGCAGCAGTCCTTGGCAGAAACGCTGGCTGGGTCCCTTGGTCCATCAGGATCTTCTGGAAACGTGGCAAACTATATGGGTCAAATGGCTATGGCCATGGGAAAACTTGGCACACTCGAGAATTTCCTTCGCCAGGCTGACAATCTACGACAGCAGACCTTACATCAGATGCAACGTATCCTGACAATCCGACAAGCTGCTCGTGCGCTTCTTGCAATCCATGACTACTTTTCACGTTTGCGCGCACTGAGTTCTCTTTGGCTTGCTAGGCCACGAGAG